Tunable Hybrid Biopolymeric Hydrogel Scaffolds Based on Atomic Force Microscopy Characterizations for Tissue Engineering

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	Tunable Hybrid Biopolymeric Hydrogel Scaffolds Based on Atomic Force Microscopy Characterizations for Tissue Engineering
	Li M(李密)2,3 ; Wang YC(王越超)2,3 ; Xi N(席宁)1 ; Liu LQ(刘连庆)2,3
刊名	IEEE Transactions on Nanobioscience
	2019
卷号	18 期号:4 页码:597-610
关键词	Atomic force microscopy hydrogel scaffolds natural biopolymers peak force tapping cellular spheroids tissue engineering
ISSN号	1536-1241
产权排序	1
英文摘要	Developing adequate biomaterials to engineer cell-scaffold interactions has become a promising way for physically regulating the biological behaviors of cells in the field of tissue engineering. Biopolymeric hydrogels have shown great merits as cellular scaffolds due to their biocompatible and biodegradable characteristics. In particular, the advent of atomic force microscopy (AFM) provides a powerful tool for characterizing native specimens at the micro/nanoscale, but utilizing AFM to investigate the detailed structures and properties of hydrogel scaffolds has been still scarce. In this paper, hybrid natural biopolymers are used to form hydrogel scaffolds which exhibit tunable structural and mechanical properties characterized by AFM peak force tapping imaging, and the applications of the formed hydrogel scaffolds in tissue engineering are studied. AFM morphological images showed that the cross-linking reactions of sodium alginate and gum arabic via calcium cations yielded the porous hydrogel scaffolds. By altering the component ratios, AFM mechanical images showed that the porous and mechanical properties (Young's modulus and adhesion force) of the hydrogel scaffolds were tunable. Next, the nanoscale structural and mechanical dynamics of the fabricated hydrogel scaffolds during the degradation process were revealed by AFM peak force tapping imaging. The experimental results on three different types of cells showed that the fabricated hydrogel scaffolds facilitate the formation of cellular spheroids. The research provides a novel idea to design tunable hydrogel scaffolds based on AFM characterizations for investigating cell-scaffold interactions, which will have potential impacts on tissue engineering.
语种	英语
资助机构	National Natural Science Foundation of China under Grant 61873258, Grant 61503372, and Grant U1613220
内容类型	期刊论文
源URL	[http://ir.sia.cn/handle/173321/25806]
专题	沈阳自动化研究所_机器人学研究室
作者单位	1.Department of Industrial and Manufacturing Systems Engineering, University of Hong Kong, Hong Kong, Hong Kong 2.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China 3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
推荐引用方式 GB/T 7714	Li M,Wang YC,Xi N,et al. Tunable Hybrid Biopolymeric Hydrogel Scaffolds Based on Atomic Force Microscopy Characterizations for Tissue Engineering[J]. IEEE Transactions on Nanobioscience,2019,18(4):597-610.
APA	Li M,Wang YC,Xi N,&Liu LQ.(2019).Tunable Hybrid Biopolymeric Hydrogel Scaffolds Based on Atomic Force Microscopy Characterizations for Tissue Engineering.IEEE Transactions on Nanobioscience,18(4),597-610.
MLA	Li M,et al."Tunable Hybrid Biopolymeric Hydrogel Scaffolds Based on Atomic Force Microscopy Characterizations for Tissue Engineering".IEEE Transactions on Nanobioscience 18.4(2019):597-610.