Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic

	Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic
	Lu Dong 1,3,4; Zhang Miao-Miao3,4 ; Zhang Wen-De 2; Cao Guo-Zhen 1,3,4; Miao Jian-Shun 3,4; Li Wen-Jian1,3,4
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2015-12-01
卷号	26 页码:9
关键词	X-rays Saccharomyces cerevisiae Growth curve Physiological-biochemical characteristic
ISSN号	1001-8042
英文摘要	In this paper, the growth curves of yeast cells exposed to X-rays were detected, and then fitted by Gompertz equation. The yeast cells treated with 50-125 Gy showed an increased exponential growth rate, and lower total biomass at plateau. At doses >= 150 Gy, cells showed a decreased exponential growth rate and higher total biomass at plateau. DNA lesions were detected by comet assay. Meanwhile, intracellular accumulation of reactive oxygen species (ROS), reduction of mitochondrial membrane potential (Delta Psi m) and cell membrane integrity were evaluated. We conclude that X-ray irradiation results in DNA lesions, ROS accumulation and Delta Psi m decline in a dose-dependent manner, and that these changes may be one of causes of X-rays-induced apoptosis in yeast. Furthermore, yeast cell membrane integrity appeared compromised following irradiation, suggesting that membrane damage may also have a role in the biological effects of radiation.
资助项目	project of western talent training program of Chinese Academy of Sciences[Y306010XB0]
WOS关键词	IONIZING-RADIATION ; DEPENDENT APOPTOSIS ; TRACK STRUCTURE ; YEAST ; DNA ; MEMBRANE ; STRESS ; RESPONSES ; REPAIR ; DAMAGE
WOS研究方向	Nuclear Science & Technology ; Physics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000368964600008
资助机构	project of western talent training program of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/41538]
专题	近代物理研究所_生物物理研究室
通讯作者	Lu Dong
作者单位	1.Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China 2.Gansu Ronghua Ind Grp Co Ltd, Wuwei 733000, Peoples R China 3.Key Lab Microbial Resources Exploitat & Applicat, Lanzhou 730000, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Lu Dong,Zhang Miao-Miao,Zhang Wen-De,et al. Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic[J]. NUCLEAR SCIENCE AND TECHNIQUES,2015,26:9.
APA	Lu Dong,Zhang Miao-Miao,Zhang Wen-De,Cao Guo-Zhen,Miao Jian-Shun,&Li Wen-Jian.(2015).Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic.NUCLEAR SCIENCE AND TECHNIQUES,26,9.
MLA	Lu Dong,et al."Impacts of X-ray irradiation on Saccharomyces cerevisiae cells growth and physiological-biochemical characteristic".NUCLEAR SCIENCE AND TECHNIQUES 26(2015):9.