Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect

doi:10.1080/15476286.2015.1100795

	Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect
	Ding, Nan1 ; Zhu, Qiyun 2; Wei, Wenjun 1; Hua, Junrui1 ; Wang, Bing1 ; Zhang, Xurui 1; Hu, Wentao 1; Wang, Jufang1 ; Xu, Shuai 1,2
刊名	RNA BIOLOGY
	2015-12-02
卷号	12 页码:1355-1363
关键词	bystander effect exosome microRNA miR-21 radiation
ISSN号	1547-6286
DOI	10.1080/15476286.2015.1100795
英文摘要	Bystander effects can be induced through cellular communication between irradiated cells and non-irradiated cells. The signals that mediate this cellular communication, such as cytokines, reactive oxygen species, nitric oxide and even microRNAs, can be transferred between cells via gap junctions or extracellular medium. We have previously reported that miR-21, a well described DDR (DNA damage response) microRNA, is involved in radiation-induced bystander effects through a medium-mediated way. However, the mechanisms of the microRNA transfer have not been elucidated in details. In the present study, it was found that exosomes isolated from irradiated conditioned medium could induce bystander effects. Furthermore, we demonstrated plenty of evidences that miR-21, which is up-regulated as a result of mimic transfection or irradiation, can be transferred from donor or irradiated cells into extracellular medium and subsequently get access to the recipient or bystander cells through exosomes to induce bystander effects. Inhibiting the miR-21 expression in advance can offset the bystander effects to some extent. From all of these results, it can be concluded that the exosome-mediated microRNA transfer plays an important role in the radiation-induced bystander effects. These findings provide new insights into the functions of microRNAs and the cellular communication between the directly irradiated cells and the non-irradiated cells.
资助项目	National Natural Science Foundation of China[31270895] ; National Natural Science Foundation of China[31400723] ; National Natural Science Foundation of China[11405235]
WOS关键词	IONIZING-RADIATION ; DIAGNOSTIC BIOMARKERS ; CELL COMMUNICATION ; CANCER ; RNA ; MICROVESICLES ; MECHANISM ; GROWTH ; SPLEEN
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000368027500008
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/41407]
专题	近代物理研究所_生物物理研究室
通讯作者	Wang, Jufang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Gansu Key Lab Space Radiobiol, Lanzhou, Peoples R China 2.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Ding, Nan,Zhu, Qiyun,Wei, Wenjun,et al. Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect[J]. RNA BIOLOGY,2015,12:1355-1363.
APA	Ding, Nan.,Zhu, Qiyun.,Wei, Wenjun.,Hua, Junrui.,Wang, Bing.,...&Xu, Shuai.(2015).Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect.RNA BIOLOGY,12,1355-1363.
MLA	Ding, Nan,et al."Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect".RNA BIOLOGY 12(2015):1355-1363.