Serum microRNA as noninvasive indicator for space radiation

doi:10.1016/j.actaastro.2018.07.038

	Serum microRNA as noninvasive indicator for space radiation
	Wei, Wenjun 1,2; Wang, Jufang1,2 ; He, Jinpeng1,2 ; Xie, Xiaodong 3
刊名	ACTA ASTRONAUTICA
	2018-11
卷号	152 页码:101-104
关键词	Serum microRNA Ionizing radiation Indicators
ISSN号	0094-5765
DOI	10.1016/j.actaastro.2018.07.038
英文摘要	Ionizing radiation in space is one of the major barriers to long-term or deep-space exploration for human. Although physical dosimeters and biomarkers have been developed to assess the exposure risk during the space flight, there are some limitations existing in these methods such as lack of individual specificity and time-consuming. Serum microRNAs (miRNAs) are ideal biomarkers because they are stable in responding to changes of environments, conservative in different species and easy for collection and detection. The purpose of this study was to identify a set of serum miRNAs which could serve as convenient and sensitive indicators to evaluate the exposure risk of ionizing radiation in space. Eight-week-old Kunming mice were total-body exposed to different doses of carbon ions and X-rays. Serum samples were collected at 24 h after exposure, and miRNAs associated with immune system and hematopoietic system were detected by miRNA PCR array. The serum miRNA expression profiles showed that the levels of 2 miRNAs (let-7a-5p, miR-200b-5p) in irradiated mice were 2 times higher than that in non-irradiated mice (P < 0.05). Furthermore, significant dose-and time-dependence in let-7a and miR-200b were observed by quantitative-RT-PCR (q-RT PCR). The increases of miRNA levels were detectable from 0.1 to 2 Gy for carbon ions and 0.5-4 Gy for X-rays, from 6 to 72 h for both carbon ions and Xrays. These results suggest that some specific serum miRNAs have the potential of serving as novel noninvasive indicators for space radiation.
资助项目	International Science & Technology Cooperation Program of China[2015DFR30940]
WOS关键词	BIODOSIMETRY ; IDENTIFICATION ; LYMPHOCYTES ; BIOMARKERS
WOS研究方向	Engineering
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000449235500010
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/64415]
专题	中国科学院近代物理研究所
通讯作者	Wang, Jufang; Xie, Xiaodong
作者单位	1.Chinese Acad Sci, Key Lab Space Radiobiol Gansu Prov, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Heavy Ion Radiat Biol & Med, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 3.Lanzhou Univ, Sch Basic Med Sci, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Wei, Wenjun,Wang, Jufang,He, Jinpeng,et al. Serum microRNA as noninvasive indicator for space radiation[J]. ACTA ASTRONAUTICA,2018,152:101-104.
APA	Wei, Wenjun,Wang, Jufang,He, Jinpeng,&Xie, Xiaodong.(2018).Serum microRNA as noninvasive indicator for space radiation.ACTA ASTRONAUTICA,152,101-104.
MLA	Wei, Wenjun,et al."Serum microRNA as noninvasive indicator for space radiation".ACTA ASTRONAUTICA 152(2018):101-104.