Long-term Autophagy and Nrf2 Signaling in the Hippocampi of Developing Mice after Carbon Ion Exposure

doi:10.1038/srep18636

	Long-term Autophagy and Nrf2 Signaling in the Hippocampi of Developing Mice after Carbon Ion Exposure
	Ye, Fei1,2,3,4 ; Zhao, Ting 1,4; Liu, Xiongxiong 1,4; Jin, Xiaodong1,4 ; Liu, Xinguo1,4 ; Wang, Tieshan 2; Li, Qiang1,4
刊名	SCIENTIFIC REPORTS
	2015-12-22
卷号	5
ISSN号	2045-2322
DOI	10.1038/srep18636
英文摘要	To explore charged particle radiation-induced long-term hippocampus damage, we investigated the expression of autophagy and antioxidant Nrf2 signaling-related proteins in the mouse hippocampus after carbon ion radiation. Heads of immature female Balb/c mice were irradiated with carbon ions of different LETs at various doses. Behavioral tests were performed on the mice after maturation. Acute and chronic expression of LC3-II, p62/SQSTM1, nuclear Nrf2, activated caspase-3 and the Bax/Bcl-2 ratio were measured in the hippocampi. Secondary X-ray insult was adopted to amplify potential damages. Long-term behavioral changes were observed in high-LET carbon ion-irradiated mice. There were no differences in the rates of LC3-II induction and p62/SQSTM1 degradation compared to the control group regardless of whether the mice received the secondary X-ray insult. A high nuclear Nrf2 content and low apoptosis level in hippocampal cells subjected to secondary X-rays were observed for the mice exposed to relatively low-LET carbon ions. Therefore, carbon ion exposure in the immature mouse led to an LET-dependent behavioral change after maturation. Although autophagy was intact, the persistently high nuclear Nrf2 content in the hippocampus might account for the unchanged behavioral pattern in mice exposed to the relatively low-LET carbon ions and the subsequent increased radioresistance of the hippocampus.
资助项目	National Natural Science Foundation of China[U1232207] ; National Natural Science Foundation of China[10905080] ; National Natural Science Foundation of China[11075191] ; National Natural Science Foundation of China[11205217]
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000209880900001
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/56665]
专题	中国科学院近代物理研究所
通讯作者	Li, Qiang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Dept Modern Phys, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Ye, Fei,Zhao, Ting,Liu, Xiongxiong,et al. Long-term Autophagy and Nrf2 Signaling in the Hippocampi of Developing Mice after Carbon Ion Exposure[J]. SCIENTIFIC REPORTS,2015,5.
APA	Ye, Fei.,Zhao, Ting.,Liu, Xiongxiong.,Jin, Xiaodong.,Liu, Xinguo.,...&Li, Qiang.(2015).Long-term Autophagy and Nrf2 Signaling in the Hippocampi of Developing Mice after Carbon Ion Exposure.SCIENTIFIC REPORTS,5.
MLA	Ye, Fei,et al."Long-term Autophagy and Nrf2 Signaling in the Hippocampi of Developing Mice after Carbon Ion Exposure".SCIENTIFIC REPORTS 5(2015).