Isoliquiritigenin Enhances Radiosensitivity of HepG2 Cells via Disturbance of Redox Status

doi:10.1007/s12013-012-9447-x

	Isoliquiritigenin Enhances Radiosensitivity of HepG2 Cells via Disturbance of Redox Status
	Sun, Chao 1,2,3,4; Zhang, Hong1,2,3 ; Ma, Xiao-fei 1,2,3,4; Zhou, Xin1,2,3,4 ; Gan, Lu1,2,3 ; Liu, Yuan-yuan1,2,3 ; Wang, Zhen-hua1,2,3
刊名	CELL BIOCHEMISTRY AND BIOPHYSICS
	2013-04-01
卷号	65 页码:433-444
关键词	Radox Status Ros Isoliquiritigenin Radiosensitization Nrf-2
ISSN号	1085-9195
DOI	10.1007/s12013-012-9447-x
文献子类	Article
英文摘要	Redox balance plays an important role in the maintenance of cell growth and survival. Disturbance of this equilibrium can alter normal cellular processes. Excessive reactive oxygen species (ROS) are often found in cancer cells. However, cancer cells have an efficient antioxidant system to counteract the increased generation of ROS. This high antioxidant capacity also favors resistance to drugs and radiation. Here, we show that isoliquiritigenin (ISL), a natural antioxidant, effectively decreased ROS in HepG2 cells in a time-dependant manner at 0.5, 1, and 2 h of treatment. The decreased ROS caused redox imbalance and reductive stress. To adapt to this state, nuclear factor erythroid-2-related factor 2, which regulates the antioxidant enzyme system, was significantly decreased. Antioxidant enzymes reached their lowest level at 6 h after ISL treatment. Endogenous ROS were still being generated so after 6 h of ISL treatment, ROS were clearly higher than before ISL treatment, causing redox imbalance in the HepG2 cells which changed from reductive to oxidative stress. At this stage, cells were irradiated with X-rays. The excess ROS induced serious oxidative stress, resulting in radiosensitization. Therefore, we concluded that ISL induced oxidative stress by disturbing the redox status and ultimately enhancing the radiosensitivity of HepG2 cells.
资助项目	Western Talent Program of Chinese Academy of Sciences[Y10180XB0]
WOS关键词	OXIDATIVE STRESS ; CANCER-CELLS ; DNA-DAMAGE ; IONIZING-RADIATION ; FREE-RADICALS ; ANTICANCER PROPERTIES ; CARCINOMA CELLS ; HUMAN-DISEASE ; IN-VIVO ; MECHANISMS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种	英语
出版者	HUMANA PRESS INC
WOS记录号	WOS:000316350000014
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/48119]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100000, Peoples R China
推荐引用方式 GB/T 7714	Sun, Chao,Zhang, Hong,Ma, Xiao-fei,et al. Isoliquiritigenin Enhances Radiosensitivity of HepG2 Cells via Disturbance of Redox Status[J]. CELL BIOCHEMISTRY AND BIOPHYSICS,2013,65:433-444.
APA	Sun, Chao.,Zhang, Hong.,Ma, Xiao-fei.,Zhou, Xin.,Gan, Lu.,...&Wang, Zhen-hua.(2013).Isoliquiritigenin Enhances Radiosensitivity of HepG2 Cells via Disturbance of Redox Status.CELL BIOCHEMISTRY AND BIOPHYSICS,65,433-444.
MLA	Sun, Chao,et al."Isoliquiritigenin Enhances Radiosensitivity of HepG2 Cells via Disturbance of Redox Status".CELL BIOCHEMISTRY AND BIOPHYSICS 65(2013):433-444.