A gold nanocluster-based ratiometric fluorescent probe for cysteine and homocysteine detection in living cells

doi:10.1039/c6nj04134e

	A gold nanocluster-based ratiometric fluorescent probe for cysteine and homocysteine detection in living cells
	Yu, Hui 1,2,3; Liu, Yan4 ; Wang, Jiamin 1,2,3; Liang, Qing 1,2; Liu, Hong 1,2; Xu, Jian 1,2; Shao, Shijun 1,2
刊名	NEW JOURNAL OF CHEMISTRY
	2017-06-07
卷号	41 页码:4416-4423
ISSN号	1144-0546
DOI	10.1039/c6nj04134e
英文摘要	A novel organic-inorganic ratiometric fluorescent probe AuNCs-NBD was developed for the detection of cysteine (Cys) and homocysteine (Hcy). In our design, the AuNCs-NBD exhibited dual-emission fluorescence properties with a single excitation. Bovine serum albumin (BSA) protected gold nanoclusters (AuNCs) with carboxyl groups were employed as a reference signal unit and conjugated with the p-aminothiophenol substituted NBD (7-nitro-2,1,3-benzoxadiazole) moiety to form the ratiometric fluorescent probe. The AuNCs-NBD composite showed a single red fluorescence at 630 nm ascribed to AuNCs, and reaction of AuNCs-NBD with Cys and Hcy caused the cleavage of the thioether bond and therefore generated the fluorescent amino linked NBD with emission at 540 nm. Accordingly, the green fluorescence of released NBD at 540 nm gradually increased upon increasing the concentration of Cys or Hcy, while the red fluorescence ascribed to AuNCs at 630 nm remained constant, which resulted in the ratiometric determination of Cys and Hcy using the AuNCs-NBD probe. Due to the specific recognition of NBD with Cys and Hcy, the AuNCs-NBD probe exhibited high selectivity for Cys and Hcy over other amino acids and GSH. In addition, the AuNCs-NBD composite displayed desirable properties such as high specificity, appreciable solubility, ratiometric fluorescence response and favorable detection limit. Moreover, the AuNCs-NBD probe has been successfully applied for monitoring and imaging of intracellular Cys and Hcy in HeLa cells.
资助项目	National Natural Science Foundation of China[21572239] ; National Natural Science Foundation of China[21505145] ; National Natural Science Foundation of China[21275150] ; Personnel Training Project of the West Light Foundation of the Chinese Academy of Sciences ; Top Priority Program of "One-Three-Five" Strategic Planning of Lanzhou Institute of Chemical Physics, CAS
WOS关键词	SELECTIVE DETECTION ; IN-VIVO ; BLOOD-PLASMA ; CANCER-CELLS ; LIVE CELLS ; GLUTATHIONE ; THIOLS ; AGGREGATION ; NANOPARTICLES ; RECOGNITION
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000402377400021
资助机构	National Natural Science Foundation of China ; Personnel Training Project of the West Light Foundation of the Chinese Academy of Sciences ; Top Priority Program of "One-Three-Five" Strategic Planning of Lanzhou Institute of Chemical Physics, CAS
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/44936]
专题	中国科学院近代物理研究所
通讯作者	Xu, Jian; Shao, Shijun
作者单位	1.Chinese Acad Sci, CAS Key Lab Chem Northwestern Plant Resources, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Yu, Hui,Liu, Yan,Wang, Jiamin,et al. A gold nanocluster-based ratiometric fluorescent probe for cysteine and homocysteine detection in living cells[J]. NEW JOURNAL OF CHEMISTRY,2017,41:4416-4423.
APA	Yu, Hui.,Liu, Yan.,Wang, Jiamin.,Liang, Qing.,Liu, Hong.,...&Shao, Shijun.(2017).A gold nanocluster-based ratiometric fluorescent probe for cysteine and homocysteine detection in living cells.NEW JOURNAL OF CHEMISTRY,41,4416-4423.
MLA	Yu, Hui,et al."A gold nanocluster-based ratiometric fluorescent probe for cysteine and homocysteine detection in living cells".NEW JOURNAL OF CHEMISTRY 41(2017):4416-4423.