Loss of protein kinase 2 subunit alpha 2 (CK2 alpha') effect ram sperm function after freezing and thawing process

doi:10.1016/j.anireprosci.2017.01.017

	Loss of protein kinase 2 subunit alpha 2 (CK2 alpha') effect ram sperm function after freezing and thawing process
	He, Yuxuan 1; Li, Hongyan 2,3,4; Wang, Ke 5; Zhang, Yong 1; Zhao, Xingxu 1
刊名	ANIMAL REPRODUCTION SCIENCE
	2017-06-01
卷号	181 页码:9-15
关键词	Ram sperm Cryopreservation Motility Protein kinase 2 subunit alpha 2
ISSN号	0378-4320
DOI	10.1016/j.anireprosci.2017.01.017
英文摘要	Protein kinase 2 subunit alpha 2 (CK2 alpha'), a serine/threonine-selective protein kinase, is associated with sperm apoptosis. However, the presence of CK2 alpha' in ram sperm during the freezing-thawing process has not been previously reported. Thus, this study was conducted to determine the effect of cryopreservation on the association between CK2 alpha' and ram sperm function. Sperm variables, including sperm motility, DNA damage and acrosome integrity, were measured in fresh (F), cooled (CO) and freeze-thawed (FT) sperm. Sperm proteins and total mRNA were extracted form cells of each group, and subjected to western blot and real-time PCR analysis for detection of CK2 alpha' proteins and relative abundance of mRNA. The distribution pattern of CK2 alpha' protein in ram sperm was also monitored in each group using an immunofluorescence technique. The results provided evidence that the freeze-thaw process has an impact on ram sperm variables, and the normalized CK2 alpha' protein and relative abundance of CK2 alpha' mRNA were both significantly less in FT than F sperm. The amount of CK2 alpha' in FT extended seminal plasma was increased as compared with F samples. Furthermore, immunofluorescence revealed that CK2 alpha' was distributed throughout the acrosome of ram sperm. The association of CK2 alpha' with DNA damage and acrosome integrity was confirmed using Pearson's linear correlation. In conclusion, the understanding the molecular effects of cryopreservation on CK2 alpha' in ram sperm could provide insight into methods for improving fertility associated with frozen-thawed ram sperm. (C) 2017 Elsevier B.V. All rights reserved.
资助项目	Chinese Academy of Sciences[Y560020XB0] ; Fostering Foundation for the Excellent Ph.D. Dissertation of Gansu Agricultural University[2013002] ; Demonstration of the Transfer Key Technique in Mutton Sheep[GNSW-2014-30] ; Establishment of a Highly Efficient Breeding System for Sheep and Development and of Mutton Quality Control Technology[GNSW-2016-34]
WOS关键词	CARBON ION RADIATION ; INTERSPECIFIC HYBRIDIZATION ; DIFFERENTIAL PROTEOME ; MICE LACKING ; DNA-DAMAGE ; CRYOPRESERVATION ; QUALITY ; CK2 ; SPERMATOGENESIS ; FERTILIZATION
WOS研究方向	Agriculture ; Reproductive Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000403520900002
资助机构	Chinese Academy of Sciences ; Fostering Foundation for the Excellent Ph.D. Dissertation of Gansu Agricultural University ; Demonstration of the Transfer Key Technique in Mutton Sheep ; Establishment of a Highly Efficient Breeding System for Sheep and Development and of Mutton Quality Control Technology
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/44904]
专题	中国科学院近代物理研究所
通讯作者	Zhao, Xingxu
作者单位	1.Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 4.Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou 730000, Peoples R China 5.Anim Husb & Vet Res Inst Gansu Prov, Pingliang 744000, Peoples R China
推荐引用方式 GB/T 7714	He, Yuxuan,Li, Hongyan,Wang, Ke,et al. Loss of protein kinase 2 subunit alpha 2 (CK2 alpha') effect ram sperm function after freezing and thawing process[J]. ANIMAL REPRODUCTION SCIENCE,2017,181:9-15.
APA	He, Yuxuan,Li, Hongyan,Wang, Ke,Zhang, Yong,&Zhao, Xingxu.(2017).Loss of protein kinase 2 subunit alpha 2 (CK2 alpha') effect ram sperm function after freezing and thawing process.ANIMAL REPRODUCTION SCIENCE,181,9-15.
MLA	He, Yuxuan,et al."Loss of protein kinase 2 subunit alpha 2 (CK2 alpha') effect ram sperm function after freezing and thawing process".ANIMAL REPRODUCTION SCIENCE 181(2017):9-15.