A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish

doi:10.1016/j.bios.2016.10.050

	A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish
	Wang, Peng 1,2; Wu, Jiang 1,2; Di, Cuixia3 ; Zhou, Rong 3; Zhang, Hong3 ; Su, Pingru 1,2; Xu, Cong 1,2; Zhou, Panpan 1,2; Ge, Yushu 4; Li, Dan 4
刊名	BIOSENSORS & BIOELECTRONICS
	2017-06-15
卷号	92 页码:602-609
关键词	Peptide Fluorescence chemosensor Hydrogen sulfide detection Cell imaging Zebrafish imaging
ISSN号	0956-5663
DOI	10.1016/j.bios.2016.10.050
英文摘要	Hydrogen sulfide (H2S) plays an important role as a signaling compound (gasotransmitter) in living systems. However, the development of an efficient imaging chemosensor of H2S in live animals is a challenging field for chemists. Herein, a novel peptide-based fluorescence chemosensor L-Cu was designed and synthesized on the basis of the copper chelating with the peptide ligand (FITC-Ahx-Ser-Pro-Gly-His-NH2, L), and its H2S sensing ability has been evaluated both in living cells and zebrafish. The peptide backbone and Cu2+-removal sensing mechanism are used to deliver rapid response time, high sensitivity, and good biocompatibility. After a fast fluorescence quench by Cu2+ coordinated with L, the fluorescence of L is recovered by adding S2- to form insoluble copper sulfide in aqueous solution with a detection limit for hydrogen sulfide measured to be 31 nM. Furthermore, the fluorescence chemosensor L-Cu showed excellent cell permeation and low biotoxicity to realize the intracellular biosensing, L-Cu has also been applied to image hydrogen sulfide in live zebrafish larvae. We expect that this peptide-based fluorescence chemosensor L-Cu can be used to study H2S-related chemical biology in physiological and pathological events.
资助项目	National Natural Science Foundation of China[21471071] ; National Natural Science Foundation of China[21431002] ; National Natural Science Foundation of China[21201091] ; Fundamental Research Funds for the Central Universities[lzujbky-2016-k10] ; Fundamental Research Funds for the Central Universities[lzujbky-2016-211]
WOS关键词	LIVE CELLS ; BIOLOGICAL-SYSTEMS ; AQUEOUS-SOLUTIONS ; ZINC IONS ; PROBE ; SENSORS ; DESIGN ; H2S ; LUMINESCENCE ; INDICATOR
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000396946600083
资助机构	National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/44643]
专题	中国科学院近代物理研究所
通讯作者	Wu, Jiang; Tang, Yu
作者单位	1.Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Dept Heavy Ion Radiat Med, 509 Nanchang Rd, Lanzhou 730000, Peoples R China 4.Univ Sci & Technol China, Sch Life Sci, Collaborat Innovat Ctr Chem Life Sci, Hefei 230027, Peoples R China
推荐引用方式 GB/T 7714	Wang, Peng,Wu, Jiang,Di, Cuixia,et al. A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish[J]. BIOSENSORS & BIOELECTRONICS,2017,92:602-609.
APA	Wang, Peng.,Wu, Jiang.,Di, Cuixia.,Zhou, Rong.,Zhang, Hong.,...&Tang, Yu.(2017).A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish.BIOSENSORS & BIOELECTRONICS,92,602-609.
MLA	Wang, Peng,et al."A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish".BIOSENSORS & BIOELECTRONICS 92(2017):602-609.