Self-aspiration sampling extractive electrospray ionization mass spectrometry (EESI-MS) for high-throughput analysis of liquid samples

doi:10.1002/rcm.7616

	Self-aspiration sampling extractive electrospray ionization mass spectrometry (EESI-MS) for high-throughput analysis of liquid samples
	Jia, Bin 1,2; Zhang, Shuai 3; Yan, Ling 1,2; He, Shoubo 4; Liu, Baohong 1,2; Shen, Huali 1,2; Yang, Pengyuan 1,2
刊名	RAPID COMMUNICATIONS IN MASS SPECTROMETRY
	2016-08-01
卷号	30 页码:56-61
ISSN号	0951-4198
DOI	10.1002/rcm.7616
英文摘要	RATIONALE: Extractive electrospray ionization mass spectrometry (EESI-MS) was invented as a typical ambient mass spectrometry method (AMS) and has been used for analyzing complex liquid samples. Here, we designed a Venturi effect-based self-aspiration sampling device and applied it to the EESI-MS for high-throughput analysis of liquid sample. METHODS: A special concentric nebulizer was designed and employed to produce a suction force for the direct aspiration of liquid samples, followed by ionization and detection. This sample aspiration process was explained and optimized using computational fluid dynamics (CFD) analysis. Experiment data were recorded to exhibit the sensitivity, memory effect, inter-day reproducibility, throughput, and applicability of the self-aspiration sampling EESI-MS. RESULTS: The limit of detection (LOD) of this method was determined as 4.5 x 10(-10) g/mL (S/N = 3) for caffeine, and the sample throughput and relative standard deviation (RSD) for full scan mode can reach 0.67 samples/s and 4.76%, respectively. Even for MS/MS mode, the frequency can still be kept at 0.4 samples/s (RSD = 4.71%). Inter-day RSD examined in 1 week was below 10% for the signal of characteristic fragment ions of reserpine. Moreover, based on this method, the amount of caffeine in instant coffee was determined as 4.7%. This device was also proven to be suitable for the protein/peptide analysis. CONCLUSIONS: These experiment results, especially the amazing results on sample throughput and inter-day RSD, suggest that we provide a valuable device which can be used for the direct high-throughput qualitative/quantitative mass spectrometry analysis of real liquid samples in ambient. Copyright (C) 2016 John Wiley & Sons, Ltd.
WOS关键词	REAL-TIME ; RAPID CHARACTERIZATION ; AMBIENT CONDITIONS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000382975400012
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/43214]
专题	中国科学院近代物理研究所
通讯作者	Shen, Huali; Yang, Pengyuan
作者单位	1.Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China 2.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China 3.Fudan Univ, Sch Basic Med Sci, Dept Anat Histol & Embryol, Shanghai 200032, Peoples R China 4.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Jia, Bin,Zhang, Shuai,Yan, Ling,et al. Self-aspiration sampling extractive electrospray ionization mass spectrometry (EESI-MS) for high-throughput analysis of liquid samples[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2016,30:56-61.
APA	Jia, Bin.,Zhang, Shuai.,Yan, Ling.,He, Shoubo.,Liu, Baohong.,...&Yang, Pengyuan.(2016).Self-aspiration sampling extractive electrospray ionization mass spectrometry (EESI-MS) for high-throughput analysis of liquid samples.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,30,56-61.
MLA	Jia, Bin,et al."Self-aspiration sampling extractive electrospray ionization mass spectrometry (EESI-MS) for high-throughput analysis of liquid samples".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 30(2016):56-61.