The influence of non-DNA-targeted effects on carbon ion-induced low-dose hyper-radiosensitivity in MRC-5 cells

doi:10.1093/jrr/rrv072

	The influence of non-DNA-targeted effects on carbon ion-induced low-dose hyper-radiosensitivity in MRC-5 cells
	Ye, Fei1,2,3,4 ; Ning, Jing 5; Liu, Xinguo1,4 ; Jin, Xiaodong1,4 ; Wang, Tieshan 2; Li, Qiang1,4
刊名	JOURNAL OF RADIATION RESEARCH
	2016-03-01
卷号	57 页码:103-109
关键词	low-dose hyper-radiosensitivity non-DNA-targeted effect gap junctional intercellular communication carbon-ion radiation
ISSN号	0449-3060
DOI	10.1093/jrr/rrv072
英文摘要	Low-dose hyper-radiosensitivity (LDHRS) is a hot topic in normal tissue radiation protection. However, the primary causes for LDHRS still remain unclear. In this study, the impact of non-DNA-targeted effects (NTEs) on high-LET radiation-induced LDHRS was investigated. Human normal lung fibroblast MRC-5 cells were irradiated with high-LET carbon ions, and low-dose biological effects (in terms of various bio-endpoints, including colony formation, DNA damage and micronuclei formation) were detected under conditions with and without gap junctional intercellular communication (GJIC) inhibition. LDHRS was observed when the radiation dose was <0.2 Gy for all bio-endpoints under investigation, but vanished when the GJIC was suppressed. Based on the probability of cells being hit and micro-dose per cell calculation, we deduced that the LDHRS phenomenon came from the combined action of direct hits and NTEs. We concluded that GJIC definitely plays an important role in cytotoxic substance spreading in high-LET carbon ion-induced LDHRS.
资助项目	National Natural Science Foundation of China[U1232207] ; National Natural Science Foundation of China[10905080] ; National Natural Science Foundation of China[11075191] ; National Natural Science Foundation of China[11205217]
WOS关键词	GAP-JUNCTION COMMUNICATION ; X-RAY MICROBEAM ; V79 CELLS ; 2ND CANCERS ; HEAVY-IONS ; RADIATION ; HYPERSENSITIVITY ; IRRADIATION ; IMPACT ; PROPAGATION
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000372619800001
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/42076]
专题	中国科学院近代物理研究所
通讯作者	Li, Qiang
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Med Phys Div, Lanzhou 730000, Peoples R China 2.Lanzhou Univ, Dept Modern Phys, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou 730000, Peoples R China 5.Gansu Prov Peoples Hosp, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Ye, Fei,Ning, Jing,Liu, Xinguo,et al. The influence of non-DNA-targeted effects on carbon ion-induced low-dose hyper-radiosensitivity in MRC-5 cells[J]. JOURNAL OF RADIATION RESEARCH,2016,57:103-109.
APA	Ye, Fei,Ning, Jing,Liu, Xinguo,Jin, Xiaodong,Wang, Tieshan,&Li, Qiang.(2016).The influence of non-DNA-targeted effects on carbon ion-induced low-dose hyper-radiosensitivity in MRC-5 cells.JOURNAL OF RADIATION RESEARCH,57,103-109.
MLA	Ye, Fei,et al."The influence of non-DNA-targeted effects on carbon ion-induced low-dose hyper-radiosensitivity in MRC-5 cells".JOURNAL OF RADIATION RESEARCH 57(2016):103-109.