pATM and γH2AX are effective radiation biomarkers in assessing the radiosensitivity of 12C6+ in human tumor cells

doi:10.1186/s12935-017-0419-5

	pATM and γH2AX are effective radiation biomarkers in assessing the radiosensitivity of 12C6+ in human tumor cells
	Zhao,Jin 1; Guo,Zhong 1; Pei,Shuyan 1; Song,Lei 1; Wang,Chenjing 1; Ma,Jianxiu 1; Jin,Long 1; Ma,Yanqing 1; He,Renke 1; Zhong,Jianbin 1
刊名	Cancer Cell International
	2017-04-26
卷号	17
关键词	12C6+ Human tumor cells Survival fraction γH2AX ATM
ISSN号	1475-2867
DOI	10.1186/s12935-017-0419-5
英文摘要	AbstractBackgroundTumour radiosensitivity would be particularly useful in optimizing the radiation dose during radiotherapy. The aim of the current study was to evaluate the potential value of phosphorylated H2AX (γH2AX) and ATM (pATM) in assessing 12C6+ radiosensitivity of tumour cells.MethodsHuman cervical carcinoma HeLa cells, hepatoma HepG2 cells, and mucoepidermoid carcinoma MEC-1 cells were irradiated with different doses of 12C6+. The survival fraction was assayed with clonogenic survival method and the foci of γH2AX and pATM was visualized using immunocytochemical methods. Flow cytometry was used to assay γH2AX, pATM and the cell cycle.ResultsThe survival fraction decreased immediately in dose-dependent manner, but in turn, significantly increased during 24?h after 12C6+ irradiation. Both γH2AX and pATM foci accumulated linearly with doses and with a maximum induction at 0.5?h for γH2AX and 0.5 or 4?h for pATM, respectively, and a fraction foci kept for 24?h. The expression of γH2AX and pATM was in relation to cell cycle. The G0/G1 phase cells had the highest expression of γH2AX after 0.5?h irradiation and then decreased to a lower level at 24?h after irradiation. An obvious increase of pATM in G2/M phase was shown after 24?h of 2 and 4?Gy irradiation. The significant G2/M phase arrest was shown. There was a close relationship between the clonogenic survival and γH2AX and pATM expression both in timing and dose in response to 12C6+.ConclusionsThe rate of γH2AX and pATM formation and loss may be an important factor in the response of cells to 12C6+. pATM and γH2AX are effective radiation biomarkers in assessing the radiosensitivity of 12C6+ in human tumor cells.
资助项目	National Undergraduate Training Programs for Innovation and Entrepreneurship[201610742002]
WOS关键词	DOUBLE-STRAND BREAKS ; PHOSPHORYLATED HISTONE H2AX ; CLUSTERED DNA-DAMAGE ; CARBON ION-BEAM ; X-RAYS ; ATM ; REPAIR ; EXPRESSION ; IRRADIATION ; RESPONSES
WOS研究方向	Oncology
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/S12935-017-0419-5
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/24466]
专题	中国科学院近代物理研究所
通讯作者	Zhao,Jin
作者单位	1.Medical College of Northwest Minzu University 2.Chinese Academy of Science; Department of Radiation Medicine, Institute of Modern Physics
推荐引用方式 GB/T 7714	Zhao,Jin,Guo,Zhong,Pei,Shuyan,et al. pATM and γH2AX are effective radiation biomarkers in assessing the radiosensitivity of 12C6+ in human tumor cells[J]. Cancer Cell International,2017,17.
APA	Zhao,Jin.,Guo,Zhong.,Pei,Shuyan.,Song,Lei.,Wang,Chenjing.,...&Zhang,Hong.(2017).pATM and γH2AX are effective radiation biomarkers in assessing the radiosensitivity of 12C6+ in human tumor cells.Cancer Cell International,17.
MLA	Zhao,Jin,et al."pATM and γH2AX are effective radiation biomarkers in assessing the radiosensitivity of 12C6+ in human tumor cells".Cancer Cell International 17(2017).