The effect of microdosimetric 12C6+ heavy ion irradiation and Mg2+ on canthaxanthin production in a novel strain of Dietzia natronolimnaea

doi:10.1186/1471-2180-13-213

	The effect of microdosimetric 12C6+ heavy ion irradiation and Mg2+ on canthaxanthin production in a novel strain of Dietzia natronolimnaea
	Zhou,Xiang 1; Xie,Jia-Rong 2; Tao,Lei 3; Xin,Zhi-Jun 1; Zhao,Feng-Wu 3; Lu,Xi-Hong 1; Zhao,Mei-Rong 1; Wang,Liang 1; Liang,Jian-Ping 1
刊名	BMC Microbiology
	2013-09-28
卷号	13
关键词	D. natronolimnaea svgcc1.2736 Microdosimetric 12C6+-ions Irradiation Productivity Canthaxanthin Response surface methodology
ISSN号	1471-2180
DOI	10.1186/1471-2180-13-213
英文摘要	AbstractBackgroundDietzia natronolimnaea is one of the most important bacterial bioresources for high efficiency canthaxanthin production. It produces the robust and stable pigment canthaxanthin, which is of special interest for the development of integrated biorefineries. Mutagenesis employing 12C6+ irradiation is a novel technique commonly used to improve microorganism productivity. This study presents a promising route to obtaining the highest feasible levels of biomass dry weight (BDW), and total canthaxanthin by using a microdosimetric model of 12C6+ irradiation mutation in combination with the optimization of nutrient medium components.ResultsThis work characterized the rate of both lethal and non-lethal dose mutations for 12C6+ irradiation and the microdosimetric kinetic model using the model organism, D. natronolimnaea svgcc1.2736. Irradiation with 12C6+ ions resulted in enhanced production of canthaxanthin, and is therefore an effective method for strain improvement of D. natronolimnaea svgcc1.2736. Based on these results an optimal dose of 0.5–4.5 Gy, Linear energy transfer (LET) of 80 keV μm-1and energy of 60 MeV u-1 for 12C6+ irradiation are ideal for optimum and specific production of canthaxanthin in the bacterium. Second-order empirical calculations displaying high R-squared (0.996) values between the responses and independent variables were derived from validation experiments using response surface methodology. The highest canthaxanthin yield (8.14 mg) was obtained with an optimized growth medium containing 21.5 g L-1 D-glucose, 23.5 g L-1 mannose and 25 ppm Mg2+ in 1 L with an irradiation dose of 4.5 Gy.ConclusionsThe microdosimetric 12C6+ irradiation model was an effective mutagenic technique for the strain improvement of D. natronolimnaea svgcc1.2736 specifically for enhanced canthaxanthin production. At the very least, random mutagenesis methods using 12C6+ions can be used as a first step in a combined approach with long-term continuous fermentation processes. Central composite design-response surface methodologies (CCD-RSM) were carried out to optimize the conditions for canthaxanthin yield. It was discovered D-glucose, Mg2+ and mannose have significant influence on canthaxanthin biosynthesis and growth of the mutant strain.
资助项目	Hundred Talent Program of the Chinese Academy of Science[O861010ZYO]
WOS关键词	RESPONSE-SURFACE METHODOLOGY ; PROTEIN CROSS-LINKS ; FED-BATCH PROCESS ; BIODIESEL PRODUCTION ; ENHANCED PRODUCTION ; BETA-CAROTENE ; KINETIC-MODEL ; OPTIMIZATION ; RADIATION ; HS-1
WOS研究方向	Microbiology
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/1471-2180-13-213
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/24450]
专题	中国科学院近代物理研究所
通讯作者	Zhou,Xiang; Liang,Jian-Ping
作者单位	1.Chinese Academy of Sciences; Institute of Modern Physics 2.China Pharmaceutical University 3.Lanzhou Vocational Technical College
推荐引用方式 GB/T 7714	Zhou,Xiang,Xie,Jia-Rong,Tao,Lei,et al. The effect of microdosimetric 12C6+ heavy ion irradiation and Mg2+ on canthaxanthin production in a novel strain of Dietzia natronolimnaea[J]. BMC Microbiology,2013,13.
APA	Zhou,Xiang.,Xie,Jia-Rong.,Tao,Lei.,Xin,Zhi-Jun.,Zhao,Feng-Wu.,...&Liang,Jian-Ping.(2013).The effect of microdosimetric 12C6+ heavy ion irradiation and Mg2+ on canthaxanthin production in a novel strain of Dietzia natronolimnaea.BMC Microbiology,13.
MLA	Zhou,Xiang,et al."The effect of microdosimetric 12C6+ heavy ion irradiation and Mg2+ on canthaxanthin production in a novel strain of Dietzia natronolimnaea".BMC Microbiology 13(2013).