With the expansion of research fields and rapid increase of scientific literatures, knowledge structures of subjects become more and more complex. Subject profile can reveal the multi-level and multidimensional knowledge structures of subjects like user profile characterizes users. As a new knowledge organization technology, knowledge graph can be used to construct subject profile. Stem cell and regenerative medicine is the frontier of life science, and it is expected to become the third way to treat diseases after drug therapy and surgery. Based on multi-source scientific literatures and information, a domain knowledge graph of stem cell was constructed. Based on the knowledge graph, we are designing and constructing subject profile of stem cell, which is of great significance to help decision-makers and researchers to grasp the overall research of stem cell at different levels and perspectives.