Interferon regulatory factor 4b (IRF4b) in Japanese flounder, Paralichthys olivaceus: Sequencing, ubiquitous tissue distribution and inducible expression by poly(I:C) and DNA virus

doi:10.1016/j.dci.2016.04.012

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	Interferon regulatory factor 4b (IRF4b) in Japanese flounder, Paralichthys olivaceus: Sequencing, ubiquitous tissue distribution and inducible expression by poly(I:C) and DNA virus
	Liu, Dahai 1; Chen, Jinjing 2; Zhang, Haiyan 2; Hu, Mengzhu 2; Lou, Huimin 2; Liu, Qiuming 2; Zhang, Shicui 2,3; Hu, Guobin 2,3
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2016-09
卷号	62 页码:127-133
关键词	IRF4b Paralichthys olivaceus Viral challenges Gene expression Immune responses
ISSN号	0145-305X
DOI	10.1016/j.dci.2016.04.012
英文摘要	Interferon regulatory factor 4 (IRF4) in mammals is known to be critical in regulation of development and functions of lymphomyeloid cell lineages. Recent studies have demonstrated its involvement in immune responses to bacterial and viral challenges in teleosts. In this study, an IRF4 gene was cloned from Japanese flounder (Paralichthys olivaceus) and its expression in response to polyinosinic:polycytidylic acid [poly(I:C)] and lymphocystis disease virus (LCDV) stimulations was studied in vivo. The cloned gene spans over 5.9 kb, comprises eight exons and seven introns and encodes a putative protein of 456 amino acids. The deduced amino acid sequence possesses a conserved DNA-binding domain (DBD), an IRFassociation domain (IAD) and a nuclear localization signal (NLS). Phylogenetic analysis clustered it into the teleost IRF4b Glade and, thus, it was named Paralichthys olivaceus (Po)IRF4b. The constitutive expression of PoIRF4b transcripts was detectable in all examined organs, with highest levels found in lymphomyeloid-rich tissues. They were induced by both poly(I:C) and LCDV with a similar inducibility in immune or non-immune organs. Two waves of induced expression of PoIRF4b were observed with the two stimuli during a 7-day time course in the immune organs, with the early-phase induction being stronger. The maximum increases of PoIRF4b transcript levels ranged from 1.3 to 4.0-fold and appeared at day 1-5 post-injection depending on different organs and stimuli. In both stimulation cases, the strongest induction was detected in spleen and the weakest in muscle. These results indicate that PoIRF4b may participate in regulation of immune responses of flounders to both RNA and DNA virus infections. (C) 2016 Elsevier Ltd. All rights reserved.
资助项目	Shandong Provincial Natural Science Foundation[ZR2013CM045]
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000378456900015
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3365]
专题	业务部门_海洋政策研究中心
作者单位	1.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Peoples R China; 2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China; 3.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Liu, Dahai,Chen, Jinjing,Zhang, Haiyan,et al. Interferon regulatory factor 4b (IRF4b) in Japanese flounder, Paralichthys olivaceus: Sequencing, ubiquitous tissue distribution and inducible expression by poly(I:C) and DNA virus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,62:127-133.
APA	Liu, Dahai.,Chen, Jinjing.,Zhang, Haiyan.,Hu, Mengzhu.,Lou, Huimin.,...&Hu, Guobin.(2016).Interferon regulatory factor 4b (IRF4b) in Japanese flounder, Paralichthys olivaceus: Sequencing, ubiquitous tissue distribution and inducible expression by poly(I:C) and DNA virus.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,62,127-133.
MLA	Liu, Dahai,et al."Interferon regulatory factor 4b (IRF4b) in Japanese flounder, Paralichthys olivaceus: Sequencing, ubiquitous tissue distribution and inducible expression by poly(I:C) and DNA virus".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 62(2016):127-133.