Cloning and expression analysis of a Toll-like receptor 21 (TLR21) gene from turbot, Scophthalmus maximus

doi:10.1016/j.dci.2017.03.021

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	Cloning and expression analysis of a Toll-like receptor 21 (TLR21) gene from turbot, Scophthalmus maximus
	Li, Song 1,2; Wang, Guanjie 1,2; Liu, Dahai 3; Liu, Qiuming 1; Hu, Guobin 1,2
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2017-08
卷号	73 页码:163-168
关键词	TLR21 Scophthalmus maximus Poly(I:C) TRBIV CpG-ODN 2395 Gene expression
ISSN号	0145-305X
DOI	10.1016/j.dci.2017.03.021
英文摘要	Toll-like receptor 21 (TLR21) is a non-mammalian TLR recognizing unmethylated CpG DNA and considered as a functional homolog of mammalian TLR9. In the present study, a TLR21 gene was cloned from turbot, Scophthalmus maximus, its immune responsive expression was subsequently studied in vivo. The turbot (Sm)TLR21 gene is an intronless gene with a length of 3527 bp and encodes a peptide of 984 amino acids. The deduced protein possesses a signal peptide sequence, a leucine-rich repeat (LRR) domain composed of 16 LRR motifs, a transmembrane (TM) region and a Toll/interleukin-1 receptor (TIR) domain. Phylogenetic analysis grouped it with other teleost TLR21s. Quantitative real-time PCR (qPCR) analysis demonstrated the constitutive expression of SmTLR21 mRNA in all twelve examined tissues with higher levels in the lymphomyeloid-rich tissues like spleen and head kidney. Further, upon stimulation with polyinosinic: polycytidylic acid [poly(I:C)1, turbot reddish body iridovirus (TRBIV) and CpG oligodeoxynucleotides (CpG-ODN) 2395, the SmTLR21 mRNA expression was up-regulated in the gills, head kidney, spleen and muscle. The maximum increases of SmTLR21 transcript levels ranged from 1.3 to 8.1fold and appeared at 3 h to 5 day post-injection depending on different organs and stimuli. These findings suggest that SmTLR21 may play an important role in the immune responses to the infections of a broad range of pathogens that include RNA and DNA viruses and bacteria. (C) 2017 Elsevier Ltd. All rights reserved.
资助项目	Marine Public Welfare Industry of China[201505001]
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000401380400020
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3119]
专题	业务部门_海洋政策研究中心
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Yushan Rd 5, Qingdao 266003, Peoples R China; 2.Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China; 3.State Ocean Adm China, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Li, Song,Wang, Guanjie,Liu, Dahai,et al. Cloning and expression analysis of a Toll-like receptor 21 (TLR21) gene from turbot, Scophthalmus maximus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,73:163-168.
APA	Li, Song,Wang, Guanjie,Liu, Dahai,Liu, Qiuming,&Hu, Guobin.(2017).Cloning and expression analysis of a Toll-like receptor 21 (TLR21) gene from turbot, Scophthalmus maximus.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,73,163-168.
MLA	Li, Song,et al."Cloning and expression analysis of a Toll-like receptor 21 (TLR21) gene from turbot, Scophthalmus maximus".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 73(2017):163-168.