Expression of fatty acid desaturase genes and fatty acid accumulation in Chlamydomonas sp. ICE-L under salt stress

doi:10.1016/j.biortech.2013.09.027

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Expression of fatty acid desaturase genes and fatty acid accumulation in Chlamydomonas sp. ICE-L under salt stress
	An, Meiling 1; Mou, Shanli 2; Zhang, Xiaowen 2; Zheng, Zhou 1; Ye, Naihao 2; Wang, Dongsheng 3; Zhang, Wei 4; Miao, Jinlai 1
刊名	Bioresource Technology
	2013
卷号	149 页码:77-83
关键词	Fatty acids
ISSN号	09608524
DOI	10.1016/j.biortech.2013.09.027
英文摘要	The Antarctic ice microalgae Chlamydomonas sp. ICE-L which is highly resistant to salt stress holds promise in providing an alternative species for the production of microalgal oil. We studied the effects of the alga in confrontation with NaCl stress on the growth, oil yield and expression of fatty acid desaturase genes. The growth rate of Chlamydomonas sp. ICE-L decreased with the gradual increase in NaCl concentration. Interestingly, we found that the highest lipid content was achieved at 16‰ NaCl, reaching 23% (w/w). Meanwhile, the expression of δ9. ACPCiFAD increased rapidly while δ12. CiFAD, ω3. CiFAD2 and δ6. CiFAD showed a delayed elevation in response to altered salt stress. C18:3 was the dominant PUFA, which account for about 75% TFA in Chlamydomonas sp. ICE-L. Under 96‰ and 128‰ NaCl stress, the content of C20:5 almost approached that of C18:3. In contrast, low salinity enhanced the dominance of C18:3 at the expense of C20:3 and C20:5. © 2013 Elsevier Ltd.
电子版国际标准刊号	18732976
资助项目	National Marine Public Welfare Research Project[200805069]
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	Elsevier Ltd
WOS记录号	WOS:000328525300012
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/6792]
专题	业务部门_海洋生态研究中心
作者单位	1.Key Laboratory of Marine Bioactive Substance, The First Institute of Oceanography, State Oceanic Administration, Qingdao, China;; 2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China;; 3.Qingdao Agricultural University, Qingdao, China;; 4.Qingdao University of Science and Technology, Qingdao, China
推荐引用方式 GB/T 7714	An, Meiling,Mou, Shanli,Zhang, Xiaowen,et al. Expression of fatty acid desaturase genes and fatty acid accumulation in Chlamydomonas sp. ICE-L under salt stress[J]. Bioresource Technology,2013,149:77-83.
APA	An, Meiling.,Mou, Shanli.,Zhang, Xiaowen.,Zheng, Zhou.,Ye, Naihao.,...&Miao, Jinlai.(2013).Expression of fatty acid desaturase genes and fatty acid accumulation in Chlamydomonas sp. ICE-L under salt stress.Bioresource Technology,149,77-83.
MLA	An, Meiling,et al."Expression of fatty acid desaturase genes and fatty acid accumulation in Chlamydomonas sp. ICE-L under salt stress".Bioresource Technology 149(2013):77-83.