Molecular cloning and functional analysis of a voltage-gated potassium channel in lymphocytes from sea perch, Lateolabrax japonicus

doi:10.1016/j.fsi.2012.05.024

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Molecular cloning and functional analysis of a voltage-gated potassium channel in lymphocytes from sea perch, Lateolabrax japonicus
	Wang, Yongjie 1,2; Cong, Bailin 1,2; Shen, Jihong 1,2; Liu, Shenghao 1,2; Liu, Feng 1,2; Wang, Nengfei 1,2; Huang, Xiaohang 1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-09
卷号	33 期号:3 页码:605-613
关键词	Voltage-gated potassium(Kv) channels Lymphocytes LPS 4-AP Sea perch
ISSN号	1050-4648
DOI	10.1016/j.fsi.2012.05.024
英文摘要	Voltage-gated potassium (Kv) channels on cell plasma membrane play an important role in both excitable cells and non-excitable cells and Kv1 subfamily is most extensively studied channel in mammalian cells. Recently, this potassium channel was reported to control processes inside mammalian T lymphocytes such as cell proliferation and volume regulation. Little is known about Kv1 channels in fish. We have postulated the presence of such a channel in lymphocytes and speculated its potential role in immunoregulation in fish. Employing specific primers and RNA template, we cloned a segment of a novel gene from sea perch blood sample and subsequently obtained a full cDNA sequence using RACE approach. Bioinformatic analysis revealed structural and phylogenetic characteristics of a novel Kv channel gene, designated as spKv1.3, which exhibits homologous domains to the members of Kv1.3 family, but it differs notably from some other members of that family at the carboxyl terminus. Fulllength of spKv1.3 cDNA is 2152 bp with a 1440 bp open reading frame encoding a protein of 480 amino acids. SpKv1.3 gene is expressed in all of the tested organs and tissues of sea perch. To assess the postulated immune function of spKv1.3, we stimulated lymphocytes with LPS and/or channel blocker 4-AR Expression levels of messenger RNA (mRNA) of spKv1.3 under stimulation conditions were measured by quantitative RT-PCR. The results showed that LPS can motivate the up-regulation of spKv1.3 expression significantly. Interestingly, we found for the first time that 4-AP with LPS can also increase the spKv1.3 mRNA expression levels in time course. Although 4-AP could block potassium channels physically, we speculated that its effect on blockage of potassium channel may start up an alternative mechanism which feed back and evoke the spKv1.3 mRNA expression. (C) 2012 Elsevier Ltd. All rights reserved.
资助项目	Chinese National Natural Science Foundation[40876089] ; Chinese National Natural Science Foundation[41006102]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000308057700017
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4072]
专题	业务部门_海洋生态研究中心
作者单位	1.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China; 2.State Ocean Adm, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yongjie,Cong, Bailin,Shen, Jihong,et al. Molecular cloning and functional analysis of a voltage-gated potassium channel in lymphocytes from sea perch, Lateolabrax japonicus[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(3):605-613.
APA	Wang, Yongjie.,Cong, Bailin.,Shen, Jihong.,Liu, Shenghao.,Liu, Feng.,...&Huang, Xiaohang.(2012).Molecular cloning and functional analysis of a voltage-gated potassium channel in lymphocytes from sea perch, Lateolabrax japonicus.FISH & SHELLFISH IMMUNOLOGY,33(3),605-613.
MLA	Wang, Yongjie,et al."Molecular cloning and functional analysis of a voltage-gated potassium channel in lymphocytes from sea perch, Lateolabrax japonicus".FISH & SHELLFISH IMMUNOLOGY 33.3(2012):605-613.