Rapid Screening and Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database

doi:10.1016/S1872-2040(13)60665-9

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Rapid Screening and Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database
	Li Zhao-Yong 1; Chen Jun-Hui 1; Li Xin 1; Zhang Ru-Tan 1; Chen Chen 1; Shi Qian 1; Wang Shuai 1; Zheng Li 1; Wang Xiao-Ru 1,2
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2013-07
卷号	41 期号:7 页码:979-984
关键词	Red tide Paralytic shellfish poisoning Hydrophilic interaction liquid chromatography High resolution mass spectrometry Screening
ISSN号	0253-3820
DOI	10.1016/S1872-2040(13)60665-9
英文摘要	In this study, a new method was developed for the rapid screening and identification of paralytic shellfish poisoning (PSP) toxins in red tide algae by hydrophilic interaction liquid chromatography-high resolution mass spectrometry (HILIC-HR-MS) combined with accurate mass database. Toxic algae sample was extracted with 0.1 M acetic acid after cell-breaking, and then the crude extracts were analyzed by HILIC-HR-MS in positive and negative full scan modes. The accurate mass of quasi-molecular ion and main fragment ion peaks of PSP toxins in toxic algae were acquired and the PSP toxins in algae could be successfully qualitated by database screening and HR-MS data analysis using commercial software (Masshunter). The created database includes data not only the accurate relative molecular masses of 25 common PSP toxins but also the fragment ions. The results showed that the proposed HILIC-HR-ESI/MS method has high sensitive and separation efficiency. The limits of detection (LOD) of ten common PSP compounds were in the range of 10-80 nM, and it could meet the requirements for the actual screening of toxic algae samples. The developed method was applied to screen the PSP toxins in three kinds of toxic algae which demonstrated that the method was a useful tool for the rapid screening and qualitative identification of common PSP toxins in harmful algae.
资助项目	National Natural Scientific Foundation of China[20905017]
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000324468800002
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3957]
专题	业务部门_海洋生态研究中心
作者单位	1.State Ocean Adm, Res Ctr Marine Ecol, Inst Oceanog 1, Qingdao 266061, Peoples R China; 2.Xiamen Univ, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China
推荐引用方式 GB/T 7714	Li Zhao-Yong,Chen Jun-Hui,Li Xin,et al. Rapid Screening and Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2013,41(7):979-984.
APA	Li Zhao-Yong.,Chen Jun-Hui.,Li Xin.,Zhang Ru-Tan.,Chen Chen.,...&Wang Xiao-Ru.(2013).Rapid Screening and Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,41(7),979-984.
MLA	Li Zhao-Yong,et al."Rapid Screening and Identification of Paralytic Shellfish Poisoning Toxins in Red Tide Algae Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry with an Accurate-Mass Database".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 41.7(2013):979-984.