Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry

doi:10.3390/molecules20045423

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry
	Chen, Junhui 1; Shi, Qian 1,2; Wang, Yanlong 1; Li, Zhaoyong 1; Wang, Shuai 1
刊名	MOLECULES
	2015-04
卷号	20 期号:4 页码:5423-5437
关键词	ESI-TOF MS dereplication nucleobases nucleosides Syngnathus acus Linnaeus Hippocampus japonicus Kaup Anthopleura lanthogrammica Berkly
ISSN号	1420-3049
DOI	10.3390/molecules20045423
英文摘要	Nucleobase and nucleoside compounds exist widely in various organisms. An often occurring problem in the discovery of new bioactive compounds from natural products is reisolation of known nucleobase and nucleoside compounds. To resolve this problem, a capillary electrophoresis-high resolution mass spectrometry (CE-HR-MS) method providing both rapid separation and accurate mass full-scan MS data was developed for the first time to screen and dereplicate known nucleobase and nucleoside compounds in crude extracts of natural products. Instrumental parameters were optimized to obtain optimum conditions for CE separation and electrospray ionization-time-of-flight mass spectrometry (ESI-TOF/MS) detection. The proposed method was verified to be precise, reproducible, and sensitive. Using this method, known nucleobase and nucleoside compounds in different marine medicinal organisms including Syngnathus acus Linnaeus; Hippocampusjaponicus Kaup and Anthopleura lanthogrammica Berkly were successfully observed and identified. This work demonstrates that CE-HR-MS combined with an accurate mass database may be used as a powerful tool for dereplicating known nucleobase and nucleoside compounds in different types of natural products. Rapid dereplication of known nucleobase and nucleoside compounds allows researchers to focus on other leads with greater potential to yield new substances.
资助项目	National Natural Scientific Foundation of China[20905017]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000354480700012
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3681]
专题	业务部门_海洋生态研究中心
作者单位	1.State Ocean Adm, Inst Oceanog 1, Key Lab Analyt Technol Dev & Standardizat Chinese, Qingdao 266061, Peoples R China; 2.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China
推荐引用方式 GB/T 7714	Chen, Junhui,Shi, Qian,Wang, Yanlong,et al. Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry[J]. MOLECULES,2015,20(4):5423-5437.
APA	Chen, Junhui,Shi, Qian,Wang, Yanlong,Li, Zhaoyong,&Wang, Shuai.(2015).Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry.MOLECULES,20(4),5423-5437.
MLA	Chen, Junhui,et al."Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry".MOLECULES 20.4(2015):5423-5437.