Separation and purification of two minor typical diarrhetic shellfish poisoning toxins from harmful marine microalgae via combined liquid chromatography with mass spectrometric detection

doi:10.1002/jssc.201700125

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Separation and purification of two minor typical diarrhetic shellfish poisoning toxins from harmful marine microalgae via combined liquid chromatography with mass spectrometric detection
	Chen, Junhui 1,2; Wang, Yanlong 1; Pan, Lei 3; Shen, Huihui 4; Fu, Dan 1; Fu, Boqiang 5; Sun, Chengjun 1; Zheng, Li 1,2
刊名	JOURNAL OF SEPARATION SCIENCE
	2017-07
卷号	40 期号:14 页码:2906-2913
关键词	dinophysistoxin-1 harmful marine microalgae high-speed countercurrent chromatography-mass spectrometry macroporous resin okadaic acid
ISSN号	1615-9306
DOI	10.1002/jssc.201700125
英文摘要	A novel method was developed for the purification of two typical diarrhetic shellfish poisoning toxins from toxin-producing marine microalgae using macroporous resin, high-speed countercurrent chromatography-mass spectrometry, and semipreparative high-performance liquid chromatography-mass spectrometry. Analytical high-performance liquid chromatography-mass spectrometry was used for identification and purity analysis of okadaic acid and dinophysistoxin-1 because they exhibit no visible or ultraviolet absorption. First, four kinds of macroporous resins were investigated, and HP-20 macroporous resin was selected for the preenrichment and cleanup of the two target toxins. Second, the resin-purified sample was further purified using high-speed countercurrent chromatography coupled with a mass spectrometer. The purities of the obtained okadaic acid and dinophysistoxin-1 were 89.0 and 83.0%, respectively, as determined through analytical high-performance liquid chromatography-mass spectrometry. Finally, further purification was carried out using semipreparative high-performance liquid chromatography with mass spectrometry, and the purities of the final okadaic acid and dinophysistoxin-1 products were both over 98.0% based on the analytical high-performance liquid chromatography-mass spectrometry chromatograms and fraction spectra. This work demonstrates that the proposed purification process is a powerful method for the preparation of high-purity okadaic acid and dinophysistoxin-1 from toxin-producing marine microalgae. Moreover, it is particularly important for the purification and preparation of minor toxins that exhibit no visible or ultraviolet absorption from harmful marine algae.
电子版国际标准刊号	16159314
资助项目	Science & Technology Program of Lianyungang[SH1516]
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000406244500005
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3138]
专题	业务部门_海洋生态研究中心
作者单位	1.State Ocean Adm, Inst Oceanog 1, Qingdao Key Lab Analyt Technol Dev & Standardizat, 6 Xian Xia Ling Rd, Qingdao 266061, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China; 3.Qingdao Technol Univ Shandong, Coll Chem & Mol Engn, Qingdao, Peoples R China; 4.China Univ Geosci, Sch Ocean Sci, Beijing, Peoples R China; 5.Natl Inst Metrol, Lab Biol Anal & Cell Technol, Div Med & Biol Measurements, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Junhui,Wang, Yanlong,Pan, Lei,et al. Separation and purification of two minor typical diarrhetic shellfish poisoning toxins from harmful marine microalgae via combined liquid chromatography with mass spectrometric detection[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(14):2906-2913.
APA	Chen, Junhui.,Wang, Yanlong.,Pan, Lei.,Shen, Huihui.,Fu, Dan.,...&Zheng, Li.(2017).Separation and purification of two minor typical diarrhetic shellfish poisoning toxins from harmful marine microalgae via combined liquid chromatography with mass spectrometric detection.JOURNAL OF SEPARATION SCIENCE,40(14),2906-2913.
MLA	Chen, Junhui,et al."Separation and purification of two minor typical diarrhetic shellfish poisoning toxins from harmful marine microalgae via combined liquid chromatography with mass spectrometric detection".JOURNAL OF SEPARATION SCIENCE 40.14(2017):2906-2913.