A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples

doi:10.1016/j.chemosphere.2017.05.159

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples
	Jiang, Bo 1,2; Li, Guanghe 2; Xing, Yi 1; Zhang, Dayi 3; Jia, Jianli 4; Cui, Zhisong 5; Luan, Xiao 5; Tang, Hui 4
刊名	CHEMOSPHERE
	2017-10
卷号	184 页码:384-392
关键词	Genotoxicity Seawater Soil Simulation SOS model Whole-cell bioreporter
ISSN号	0045-6535
DOI	10.1016/j.chemosphere.2017.05.159
英文摘要	Whole-cell bioreporters have emerged as promising tools for genotoxicity evaluation, due to their rapidity, cost-effectiveness, sensitivity and selectivity. In this study, a method for detecting genotoxicity in environmental samples was developed using the bioluminescent whole-cell bioreporter Escherichia coli recit:luxCDABE. To further test its performance in a real world scenario, the E. coli bioreporter was applied in two cases: i) soil samples collected from chromium(VI) contaminated sites; ii) crude oil contaminated seawater collected after the Jiaozhou Bay oil spill which occurred in 2013. The chromium(VI) contaminated soils were pretreated by water extraction, and directly exposed to the bioreporter in two phases: aqueous soil extraction (water phase) and soil supernatant (solid phase). The results indicated that both extractable and soil particle fixed chromium(VI) were bioavailable to the bioreporter, and the solid-phase contact bioreporter assay provided a more precise evaluation of soil genotoxicity. For crude oil contaminated seawater, the response of the bioreporter clearly illustrated the spatial and time change in genotoxicity surrounding the spill site, suggesting that the crude oil degradation process decreased the genotoxic risk to ecosystem. In addition, the performance of the bioreporter was simulated by a modified cross-regulation gene expression model, which quantitatively described the DNA damage response of the E. coli bioreporter. Accordingly, the bioluminescent response of the bioreporter was calculated as the mitomycin C equivalent, enabling quantitative comparison of genotoxicities between different environmental samples. This bioreporter assay provides a rapid and sensitive screening tool for direct genotoxicity assessment of environmental samples. (C) 2017 Published by Elsevier Ltd.
电子版国际标准刊号	18791298
资助项目	Ministry of Science and Technology of China[2012FY130300]
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000407525500044
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/3073]
专题	业务部门_海洋生态研究中心
作者单位	1.Univ Sci & Technol Beijing, Sch Energy & Environm Engn, Beijing 100083, Peoples R China; 2.Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China; 3.Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England; 4.China Univ Min & Technol, Sch Chem & Environm Engn, Beijing 100083, Peoples R China; 5.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Bo,Li, Guanghe,Xing, Yi,et al. A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples[J]. CHEMOSPHERE,2017,184:384-392.
APA	Jiang, Bo.,Li, Guanghe.,Xing, Yi.,Zhang, Dayi.,Jia, Jianli.,...&Tang, Hui.(2017).A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples.CHEMOSPHERE,184,384-392.
MLA	Jiang, Bo,et al."A whole-cell bioreporter assay for quantitative genotoxicity evaluation of environmental samples".CHEMOSPHERE 184(2017):384-392.