Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp B-3 on gene expression in RAW264.7 macrophage cells

doi:10.1007/s13131-018-1227-z

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋生态研究中心

	Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp B-3 on gene expression in RAW264.7 macrophage cells
	Zhang Pingping 1; Li Jiang 2; Yu Leiye 3,4; Wei Jingfang 1; Xu Tong 1; Sun Guojie 1
刊名	ACTA OCEANOLOGICA SINICA
	2018-08
卷号	37 期号:8 页码:46-53
关键词	Psychrobacter sp B-3 B-3 exopolysaccharide RAW264.7 macrophage differentially expressed genes immunological pathway metabolic pathway
ISSN号	0253-505X
DOI	10.1007/s13131-018-1227-z
英文摘要	B-3 exopolysaccharide is extracted from the Antarctic psychrophilic bacterium Psychrobacter sp. B-3. We have previously shown that it activates macrophages and affects their immunoregulatory activities. To determine what genes are affected during this process, we detected the genes differentially expressed in cells of RAW264.7 macrophages treated with B-3 exopolysaccharide by transcriptomic analysis. B-3 exopolysaccharide treatment caused differential expression of 420 genes, of which 178 were up-regulated and 242 were down-regulated. These genes were shown to be involved in many aspects of cell function, mainly metabolism and immunity. Genes were enriched in multiple immune-related pathways, and the most significantly enriched genes were involved in antigen processing and presentation pathways. The pathway in which differentially expressed genes were the most significantly enriched was the metabolic pathway; specifically, the expression of many metabolic enzyme genes was altered by B-3 exopolysaccharide treatment. Additionally, the genes involved in metabolisms of amino acids, carbohydrates, lipids and nucleotides, varied to certain degrees. B-3 exopolysaccharide, therefore, appears to directly affect the immune function of RAW264.7 macrophages as an immunostimulant, or to indirectly change intracellular metabolism. This is the first study to determine the effect of an Antarctic psychrophilic bacterial exopolysaccharide on RAW264.7 macrophages. Our findings provide an important reference for research into the regulation of macrophage immune function by different polysaccharides.
资助项目	Chinese Polar Environment Comprehensive Investigation & Assessment Programs[CHINARE2017-01-05]
WOS研究方向	Oceanography
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000441239100007
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/2839]
专题	业务部门_海洋生态研究中心
作者单位	1.Hebei Univ Sci & Technol, Coll Biol Sci & Engn, Shijiazhuang 050018, Hebei, Peoples R China; 2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China; 3.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang Pingping,Li Jiang,Yu Leiye,et al. Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp B-3 on gene expression in RAW264.7 macrophage cells[J]. ACTA OCEANOLOGICA SINICA,2018,37(8):46-53.
APA	Zhang Pingping,Li Jiang,Yu Leiye,Wei Jingfang,Xu Tong,&Sun Guojie.(2018).Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp B-3 on gene expression in RAW264.7 macrophage cells.ACTA OCEANOLOGICA SINICA,37(8),46-53.
MLA	Zhang Pingping,et al."Transcriptomic analysis reveals the effect of the exopolysaccharide of Psychrobacter sp B-3 on gene expression in RAW264.7 macrophage cells".ACTA OCEANOLOGICA SINICA 37.8(2018):46-53.