Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections

doi:10.1016/j.aca.2008.02.060

CORC > 自然资源部第一海洋研究所 > 自然资源部第一海洋研究所 > 业务部门 > 海洋地质与地球物理研究室

	Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections
	Chen, Junhui 1,2; Wang, Fenymei 3; Liu, Jie 1; Lee, Frank Sen-Chun 1; Wang, Xiaoru 1; Yang, Huanghao 1
刊名	ANALYTICA CHIMICA ACTA
	2008-04-21
卷号	613 期号:2 页码:184-195
关键词	Coptis chinensis Franch alkaloids accelerated solvent extraction ultra performance liquid chromatography-tandem mass spectrometry fingerprint
ISSN号	0003-2670
DOI	10.1016/j.aca.2008.02.060
英文摘要	A new method based on accelerated solvent extraction (ASE) followed by ultra performance liquid chromatography (UPLC) analysis has been developed for the identification and quantification of major alkaloids in extracts of Coptis chinensis Franch. The UPLC system consisted of a dual detection system of photodiode array detector (PDA) and positive ion electrospray ionization-tandem mass spectrometry (ESI-MS/MS) in sequential configuration. The operational parameters of ASE including extraction solvent, extraction temperature, static extraction time and extraction cycles were optimized. UPLC analysis was performed on an ACQUITY UPLC BEH C-18 column eluted by a mobile phase of acetonitrile spiked with a buffer solution consisting of 0.50% acetic acid and 20 mmol L-1 ammonium acetate. A tandem quadrupole spectrometer operating in either full scan mode or in MS/MS mode for multiple reaction monitoring (MRM) was used for the identification and quantitative analysis of eight major alkaloids in C. chinensis Franch extracts. The samples were also analyzed on a high-performance liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (HPLC-ESI-TOF-MS) system to confirm the identification results. Three of the eight major alkaloids, berberine, palmatine and jatrorrhizine were quantified by UPLC-PDA and UPLC-MS/MS. The results indicated that both UPLC-PDA and UPLC-MS/MS methods were simple, sensitive and reliable for the determination of alkaloids in C. chinensis Franch. Seven Huanglian samples from different locations were analyzed using the established methods. UPLC fingerprints based on the distribution of the eight major alkaloids can serve as a rapid and reliable method for the authentication and quality evaluation of traditional Chinese medicine (TCM) herbs. (C) 2008 Elsevier B.V. All rights reserved.
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000255469700008
内容类型	期刊论文
源URL	[http://ir.fio.com.cn/handle/2SI8HI0U/4490]
专题	业务部门_海洋地质与地球物理研究室
作者单位	1.First Inst Oceanog SOA, QingDao Key Lab Analyt Technol Dev & Standardizat, Qingdao 266061, Peoples R China; 2.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266033, Peoples R China; 3.Shandong Entry Exit Inspect & Quarantine Bur, Ctr Tech, Qingdao 266002, Peoples R China
推荐引用方式 GB/T 7714	Chen, Junhui,Wang, Fenymei,Liu, Jie,et al. Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections[J]. ANALYTICA CHIMICA ACTA,2008,613(2):184-195.
APA	Chen, Junhui,Wang, Fenymei,Liu, Jie,Lee, Frank Sen-Chun,Wang, Xiaoru,&Yang, Huanghao.(2008).Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections.ANALYTICA CHIMICA ACTA,613(2),184-195.
MLA	Chen, Junhui,et al."Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections".ANALYTICA CHIMICA ACTA 613.2(2008):184-195.