Effect of Tanshinone IIA on the Noncovalent Interaction Between Warfarin and Human Serum Albumin Studied by Electrospray Ionization Mass Spectrometry

doi:10.1016/j.jasms.2008.06.005

	Effect of Tanshinone IIA on the Noncovalent Interaction Between Warfarin and Human Serum Albumin Studied by Electrospray Ionization Mass Spectrometry
	Liu, Jie 1,2; Wang, Xiaoru 1,2,4; Cai, Zongwei 3; Lee, Frank S. C.4
刊名	JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
	2008-10
卷号	19 期号:10 页码:1568-1575
ISSN号	1044-0305
DOI	10.1016/j.jasms.2008.06.005
英文摘要	Enhanced anticoagulation and/or even bleeding are often observed when patients on long-term warfarin (WAR) therapy consumed Danshen, a well-known medicinal herb in traditional Chinese medicine (TCM). This study demonstrates that altered WAR metabolism, arising from its interaction with the active components in Danshen, played a significant role in this curative effect. Mass spectrometric techniques including ESI-ITMS (electrospray ionization ion-trap mass spectrometry) and ESI-TOF (time-of-flight)-MS have been developed for the study Of Such drug-herb interactions. The experimental approach involved a detailed analysis and comparison of WAR metabolites in vivo from blood or Urine of rats that had been orally administrated with WAR, either singly or together with the representative bioactive component of Danshen-lipid Soluble TIIA (Tanshinon IIA), and a study of the interaction Of human serum albumin (HSA), WAR, and water-soluble sodium tanshinone ITA sulfonate (STS) in vitro. Results demonstrate that TIIA accelerates the metabolic rate of WAR, whereas STS displaces WAR from the WAR-HSA complex, resulting in an increase of free WAR concentration in blood. It is Suggested that the elevated level and enhanced metabolism of WAR is responsible for the over-anticoagulation effect observed. (J Am Soc Mass Spectrom 2008, 19, 1568-1575) (C) 2008 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry
资助项目	Research Grant Council, University Grants Committee of Hong Kong[HKBU2014/05M]
WOS关键词	HERB-DRUG INTERACTIONS ; PROTEIN COMPLEXES ; FLUORESCENCE SPECTROSCOPY ; 20S PROTEASOME ; BINDING-SITES ; CHINESE HERBS ; METABOLISM ; DANSHEN ; PHARMACOKINETICS ; IDENTIFICATION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000260268500023
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/27399]
专题	自然资源部第一海洋研究所
通讯作者	Wang, Xiaoru
作者单位	1.Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2.Xiamen Univ, Coll Chem & Chem Engn, Key Lab Analyt Sci MOE, Xiamen 361005, Peoples R China 3.Hong Kong Baptist Univ, Dept Chem, Kowloon, Hong Kong, Peoples R China 4.First Inst Oceanog SOA, Key Lab Analyt Technol Dev & Standardizat Chinese, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jie,Wang, Xiaoru,Cai, Zongwei,et al. Effect of Tanshinone IIA on the Noncovalent Interaction Between Warfarin and Human Serum Albumin Studied by Electrospray Ionization Mass Spectrometry[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2008,19(10):1568-1575.
APA	Liu, Jie,Wang, Xiaoru,Cai, Zongwei,&Lee, Frank S. C..(2008).Effect of Tanshinone IIA on the Noncovalent Interaction Between Warfarin and Human Serum Albumin Studied by Electrospray Ionization Mass Spectrometry.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,19(10),1568-1575.
MLA	Liu, Jie,et al."Effect of Tanshinone IIA on the Noncovalent Interaction Between Warfarin and Human Serum Albumin Studied by Electrospray Ionization Mass Spectrometry".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 19.10(2008):1568-1575.