Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry | |
Chen, Junhui1; Shi, Qian1,2; Wang, Yanlong1; Li, Zhaoyong1; Wang, Shuai1 | |
刊名 | MOLECULES |
2015-04 | |
卷号 | 20期号:4页码:5423-5437 |
关键词 | ESI-TOF MS dereplication nucleobases nucleosides Syngnathus acus Linnaeus Hippocampus japonicus Kaup Anthopleura lanthogrammica Berkly |
ISSN号 | 1420-3049 |
DOI | 10.3390/molecules20045423 |
英文摘要 | Nucleobase and nucleoside compounds exist widely in various organisms. An often occurring problem in the discovery of new bioactive compounds from natural products is reisolation of known nucleobase and nucleoside compounds. To resolve this problem, a capillary electrophoresis-high resolution mass spectrometry (CE-HR-MS) method providing both rapid separation and accurate mass full-scan MS data was developed for the first time to screen and dereplicate known nucleobase and nucleoside compounds in crude extracts of natural products. Instrumental parameters were optimized to obtain optimum conditions for CE separation and electrospray ionization-time-of-flight mass spectrometry (ESI-TOF/MS) detection. The proposed method was verified to be precise, reproducible, and sensitive. Using this method, known nucleobase and nucleoside compounds in different marine medicinal organisms including Syngnathus acus Linnaeus; Hippocampusjaponicus Kaup and Anthopleura lanthogrammica Berkly were successfully observed and identified. This work demonstrates that CE-HR-MS combined with an accurate mass database may be used as a powerful tool for dereplicating known nucleobase and nucleoside compounds in different types of natural products. Rapid dereplication of known nucleobase and nucleoside compounds allows researchers to focus on other leads with greater potential to yield new substances. |
资助项目 | National Natural Scientific Foundation of China[20905017] |
WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; HYDROPHILIC-INTERACTION CHROMATOGRAPHY ; CULTURED CORDYCEPS ; SECONDARY METABOLITES ; QUANTITATIVE-ANALYSIS ; ZONE-ELECTROPHORESIS ; PYRIMIDINE-BASES ; IDENTIFICATION ; PURINE ; MS |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
出版者 | MDPI AG |
WOS记录号 | WOS:000354480700012 |
内容类型 | 期刊论文 |
源URL | [http://ir.fio.com.cn:8080/handle/2SI8HI0U/26857] |
专题 | 自然资源部第一海洋研究所 |
通讯作者 | Chen, Junhui |
作者单位 | 1.State Ocean Adm, Inst Oceanog 1, Key Lab Analyt Technol Dev & Standardizat Chinese, Qingdao 266061, Peoples R China 2.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Junhui,Shi, Qian,Wang, Yanlong,et al. Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry[J]. MOLECULES,2015,20(4):5423-5437. |
APA | Chen, Junhui,Shi, Qian,Wang, Yanlong,Li, Zhaoyong,&Wang, Shuai.(2015).Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry.MOLECULES,20(4),5423-5437. |
MLA | Chen, Junhui,et al."Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry".MOLECULES 20.4(2015):5423-5437. |
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