Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies

doi:10.1155/2011/729216

	Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies
	Zheng, Fengrong 1; Sun, Xiuqin 1; Wu, Xing'an 2; Liu, Hongzhan 3; Li, Jiye 1; Wu, Suqi 2; Zhang, Jinxing 2
刊名	EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
	2011
页码	1-8
ISSN号	1741-427X
DOI	10.1155/2011/729216
英文摘要	Here, we report the construction of a vaccine against lymphocystis disease virus (LCDV) using nucleic acid vaccination technology. A fragment of the major capsid protein encoding gene from an LCDV isolated from China (LCDV-cn) was cloned into an eukaryotic expression vector pEGFP-N2, yielding a recombinant plasmid pEGFP-N2-LCDV-cn0.6kb. This plasmid was immediately expressed after liposomal transfer into the Japanese flounder embryo cell line. The recombinant plasmid was inoculated into Japanese flounder via two routes (intramuscular injection and hypodermic injection) at three doses (0.1, 5, and 15 mu g), and then T-lymphopoiesis in different tissues and antibodies raised against LCDV were evaluated. The results indicated that this recombinant plasmid induced unique humoral or cell-mediated immune responses depending on the inoculation route and conferred immune protection. Furthermore, the humoral immune responses and protective effects were significantly increased at higher vaccine doses via the two injection routes. Plasmid pEGFP-N2-LCDV0.6kb is therefore a promising vaccine candidate against LCDV in Japanese flounder.
资助项目	State 863 project[2006AA100309]
WOS关键词	HEMATOPOIETIC NECROSIS VIRUS ; HEMORRHAGIC SEPTICEMIA VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; CARASSIUS-AURATUS L. ; SALMON SALMO-SALAR ; RAINBOW-TROUT ; DNA VACCINATION ; PROTECTIVE IMMUNITY ; INTRAMUSCULAR INJECTION ; GLYCOPROTEIN GENE
WOS研究方向	Integrative & Complementary Medicine
语种	英语
出版者	HINDAWI PUBLISHING CORPORATION
WOS记录号	WOS:000293533600001
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26668]
专题	自然资源部第一海洋研究所
通讯作者	Sun, Xiuqin
作者单位	1.State Oceanog Adm China, Inst Oceanog 1, Qingdao 266061, Shandong, Peoples R China 2.Fourth Mil Med Univ, Dept Microbiol, Xian 710032, Shanxi Province, Peoples R China 3.Shandong Univ, Marine Coll, Weihai 264209, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Fengrong,Sun, Xiuqin,Wu, Xing'an,et al. Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2011:1-8.
APA	Zheng, Fengrong.,Sun, Xiuqin.,Wu, Xing'an.,Liu, Hongzhan.,Li, Jiye.,...&Zhang, Jinxing.(2011).Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,1-8.
MLA	Zheng, Fengrong,et al."Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE (2011):1-8.