Molecular Detection of Penicillium griseofulvum as the Coastal Pollution Indicator

doi:10.1007/s00284-010-9720-4

	Molecular Detection of Penicillium griseofulvum as the Coastal Pollution Indicator
	Shi, Zhengping 1,2; Bai, Shumeng 1; Tian, Li 1,2; Jiang, Huichao 1,2; Zhang, Jiuming 1
刊名	CURRENT MICROBIOLOGY
	2011-02
卷号	62 期号:2 页码:396-401
ISSN号	0343-8651
DOI	10.1007/s00284-010-9720-4
英文摘要	Molecular methods were carried out to detect Penicillium griseofulvum, a dominant species related to heavy metal pollution, which was screened from marine contaminated sediments. Based on differences in internal transcribed spacer (ITS) sequences of Penicillium genus and specific isoamyl alcohol oxidase (IAO) sequences, species-specific primers AS1/RS4 and IAO1/IAO2 of Penicillium griseofulvum were designed and synthesized which were then employed in optimized PCR systems. The detection sensitivities were compared through ordinary PCR and nested-PCR using two pairs of primers, respectively. Both primer pairs could exclusively amplify destined DNA fragment from contaminated environmental samples in our researches. As for primers AS1/RS4, the detection sensitivity for spores (pure spore DNA) could be 10 fg/mu l and 10 spores, respectively, and the detection sensitivity for the sediments was 10(2) spores/0.25 g sediments. While the detection sensitivity of IAO1/IAO2 primers was lower than that of AS1/RS4. Despite the difference in detection sensitivity, it is feasible that the species-specific primers could be used as probes for the detection of environmental pollution dominant species, Penicillium griseofulvum, since the frequency of occurrence and amount of this strain could preferably indicate the pollution degree.
资助项目	Ministry of Science and Technology of China[2007AA09Z435] ; Ministry of Science and Technology of China[2007AA091507] ; Ministry of Science and Technology of China[2008AA09Z407]
WOS关键词	CADMIUM
WOS研究方向	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000286205500011
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26633]
专题	自然资源部第一海洋研究所
通讯作者	Tian, Li
作者单位	1.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Shi, Zhengping,Bai, Shumeng,Tian, Li,et al. Molecular Detection of Penicillium griseofulvum as the Coastal Pollution Indicator[J]. CURRENT MICROBIOLOGY,2011,62(2):396-401.
APA	Shi, Zhengping,Bai, Shumeng,Tian, Li,Jiang, Huichao,&Zhang, Jiuming.(2011).Molecular Detection of Penicillium griseofulvum as the Coastal Pollution Indicator.CURRENT MICROBIOLOGY,62(2),396-401.
MLA	Shi, Zhengping,et al."Molecular Detection of Penicillium griseofulvum as the Coastal Pollution Indicator".CURRENT MICROBIOLOGY 62.2(2011):396-401.