The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp ICE-L

doi:10.1016/j.mrfmmm.2018.03.004

	The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp ICE-L
	An, Meiling 1; Zheng, Zhou 1,2,3; Qu, Changfeng 2,3; Wang, Xixi 2; Chen, Hao 1; Shi, Chongli 4; Miao, Jinlai 1,2,3,4
刊名	MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
	2018-05
卷号	809 页码:13-19
关键词	Chlamydomonas sp ICE-L (6-4) Photolyase DNA damage Photoreactivation
ISSN号	0027-5107
DOI	10.1016/j.mrfmmm.2018.03.004
英文摘要	The psychrophilic microalga, Chlamydomonas sp. ICE-L, isolated from floating ice in the Antarctic, one of the most highly UV exposed ecosystems on Earth, displays an efficient DNA photorepair capacity. Here, the first known (6-4) photolyase gene (6-4CiPhr) from C. sp. ICE-L was identified. The 6-4CiPhr encoded 559-amino acid polypeptide with a pI of 8.86, and had a predicted Mw of 64.2 kDa. Real-time PCR was carried out to investigate the response of 6-4CiPhr to UVB exposure. The transcription of 6-4CiPhr was up-regulated continuously within 6 h, achieving a maximum of 62.7-fold at 6 h. Expressing 6-4CiPhr in a photolyase-deficient Escherichia coli strain improved survival rate of the strain. In vitro activity assays of purified protein demonstrated that 6-4CiPhr was a photolyase with 6-4PP repair activity. These findings improve understanding of photoreactivation mechanisms of (6-4) photolyase.
资助项目	Qingdao Entrepreneurship & Innovation Pioneers Program[15-10-3-15-(44)-zch]
WOS关键词	DROSOPHILA CRYPTOCHROME ; DIMER REPAIR ; LIGHT ; FAMILY ; PHOTOPRODUCT ; MECHANISMS ; INVITRO ; TIME
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000432598800003
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26496]
专题	自然资源部第一海洋研究所
通讯作者	Miao, Jinlai
作者单位	1.Qingdao Univ, Coll Med, Qingdao 266071, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China 3.Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod Qingdao, Qingdao 266237, Peoples R China 4.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China
推荐引用方式 GB/T 7714	An, Meiling,Zheng, Zhou,Qu, Changfeng,et al. The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp ICE-L[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2018,809:13-19.
APA	An, Meiling.,Zheng, Zhou.,Qu, Changfeng.,Wang, Xixi.,Chen, Hao.,...&Miao, Jinlai.(2018).The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp ICE-L.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,809,13-19.
MLA	An, Meiling,et al."The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp ICE-L".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 809(2018):13-19.