Multipoint recognition of domoic acid from seawater by dummy template molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography

doi:10.1016/j.chroma.2017.04.023

	Multipoint recognition of domoic acid from seawater by dummy template molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography
	He, Xiuping 1,2; Chen, Jianlei 1; Wang, Jiangtao 1; Tan, Liju 1
刊名	JOURNAL OF CHROMATOGRAPHY A
	2017-06-02
卷号	1500 页码:61-68
关键词	Molecularly imprinted solid-phase extraction Dummy template Domoic acid Isolation Seawater
ISSN号	0021-9673
DOI	10.1016/j.chroma.2017.04.023
英文摘要	Due to the high cost of domoic acid (DA), different carboxylic acid compounds including indole-3-acetic acid (IAA), pyrrole-2-formic acid (PFA), pyridine-2,3-dicarboxylic anhydride (PDA), trimesitinic acid (TA) and citric acid (CA) were investigated as dummy templates for the molecularly imprinted solid-phase extraction (MISPE) for selective isolation and pre-concentration of an amnesic shellfish poison (ASP), DA. The highest binding amount of the polymers towards DA was obtained when CA was used as dummy template owing to its high hydrophilicity. In addition, the "four-point" recognition site constructed by three COOH groups and a OH group in CA was also speculated to be the reason for the high binding amount of CA-MIPs and the rebinding of DA can be depend on the three COOH groups and a NH group with conformational change in the recognition process. Finally, the CA-MISPE column was chosen for DA isolation and pre-concentration and effective result was obtained with recoveries higher than 90% and relative standard deviation (RSD) less than 5% (n =3). This new polymer can be effectively applied to the monitoring and predicting the existence of trace DA. (C) 2017 Elsevier B.V. All rights reserved.
资助项目	Assessment of Nanomaterials on Biological and Ecological Effects in Coastal Area[201505034]
WOS关键词	BIOLOGICAL SAMPLES ; POLYMERS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000401674700006
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26312]
专题	自然资源部第一海洋研究所
通讯作者	Wang, Jiangtao
作者单位	1.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	He, Xiuping,Chen, Jianlei,Wang, Jiangtao,et al. Multipoint recognition of domoic acid from seawater by dummy template molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1500:61-68.
APA	He, Xiuping,Chen, Jianlei,Wang, Jiangtao,&Tan, Liju.(2017).Multipoint recognition of domoic acid from seawater by dummy template molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1500,61-68.
MLA	He, Xiuping,et al."Multipoint recognition of domoic acid from seawater by dummy template molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1500(2017):61-68.