Temperature regulates fatty acid desaturases at a transcriptional level and modulates the fatty acid profile in the Antarctic microalga Chlamydomonas sp ICE-L

doi:10.1016/j.biortech.2013.01.142

	Temperature regulates fatty acid desaturases at a transcriptional level and modulates the fatty acid profile in the Antarctic microalga Chlamydomonas sp ICE-L
	An, Meiling 1; Mou, Shanli 2; Zhang, Xiaowen 2; Ye, Naihao 2; Zheng, Zhou 1; Cao, Shaona 2; Xu, Dong 2; Fan, Xiao 2; Wang, Yitao 2; Miao, Jinlai 1
刊名	BIORESOURCE TECHNOLOGY
	2013-04
卷号	134 页码:151-157
关键词	Chlamydomonas sp ICE-L FADs PUFAs Temperature stress
ISSN号	0960-8524
DOI	10.1016/j.biortech.2013.01.142
英文摘要	Chlamydomonas sp. ICE-L which can thrive in extreme environments of the Antarctic is a major biomass producer. The FAD genes in Chlamydomonas sp. ICE-L were obtained and sequence alignment showed that these genes are homologous to known FADs with conserved histidine motifs. In this study, we analyzed the transcription of five FADs and FA compositions at different temperatures. The results showed that the expressions of Delta 9CiFAD, omega 3CiFAD1 and omega 3CiFAD2 were apparently up-regulated at 0 degrees C, however, the up-regulation of Delta 6CiFAD intensified with rising temperature. Meanwhile, analysis of the FA compositions showed that PUFAs were dominant compositions, accounting for more than 75% TFA in Chlamydomonas sp. ICE-L Furthermore, PUFAs were significantly increased at 0 and 5 degrees C, which may be attributed to higher proportions of C18:3 and C20:3. Moreover, PUFAs were significantly decreased at 15 degrees C whereas SFAs were significantly increased. (C) 2013 Elsevier Ltd. All rights reserved.
资助项目	National Marine Public Welfare Research Project[200805069]
WOS关键词	PHOTOSYNTHETIC ENERGY-CONVERSION ; EXTREME CONDITIONS ; LIMITED GROWTH ; LIPID-CONTENT ; TOLERANCE ; PLANTS ; ACCLIMATION ; ARABIDOPSIS ; STABILITY ; DIATOMS
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000328512800012
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/26241]
专题	自然资源部第一海洋研究所
通讯作者	Ye, Naihao
作者单位	1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao, Peoples R China 2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	An, Meiling,Mou, Shanli,Zhang, Xiaowen,et al. Temperature regulates fatty acid desaturases at a transcriptional level and modulates the fatty acid profile in the Antarctic microalga Chlamydomonas sp ICE-L[J]. BIORESOURCE TECHNOLOGY,2013,134:151-157.
APA	An, Meiling.,Mou, Shanli.,Zhang, Xiaowen.,Ye, Naihao.,Zheng, Zhou.,...&Miao, Jinlai.(2013).Temperature regulates fatty acid desaturases at a transcriptional level and modulates the fatty acid profile in the Antarctic microalga Chlamydomonas sp ICE-L.BIORESOURCE TECHNOLOGY,134,151-157.
MLA	An, Meiling,et al."Temperature regulates fatty acid desaturases at a transcriptional level and modulates the fatty acid profile in the Antarctic microalga Chlamydomonas sp ICE-L".BIORESOURCE TECHNOLOGY 134(2013):151-157.