Genome-wide identification and characterization of macrolide glycosyltransferases from a marine-derived Bacillus strain and their phylogenetic distribution

doi:10.1111/1462-2920.13367

	Genome-wide identification and characterization of macrolide glycosyltransferases from a marine-derived Bacillus strain and their phylogenetic distribution
	Liu, Yang 1; Qin, Wen 1; Liu, Quanquan 1; Zhang, Jun 1; Li, Huayue 1; Xu, Shanshan 1; Ren, Pengfei 1; Tian, Li 2,3; Li, Wenli 1
刊名	ENVIRONMENTAL MICROBIOLOGY
	2016-12
卷号	18 期号:12 页码:4770-4781
ISSN号	1462-2912
DOI	10.1111/1462-2920.13367
英文摘要	Clarifying glycosyltrasferases (GTs) function is of significance for the development of GT inhibitors as drugs, and the use of GTs to glycodiversify small molecules in the search of drug leads. While many Actinomyces natural-product GTs had been functionally characterized, our understanding towards Bacillus natural-product GTs is so far very limited. Herein, genome-wide identification of macrolide GT genes from marine-derived Bacillus methylotrophicus B-9987 revealed the presence of three macrolide GT genes bmmGT123. While bmmGT1 was previously revealed to be involved in the biosynthesis of trans-acyltransferase (AT) polyketides compounds macrolactins (MLNs) and bacillaenes (BAEs), the functions of bmmGT2 and bmmGT3 were probed, demonstrating that they are capable to biochemically catalyze glycosylation of MLNs and BAEs as well but interestingly with different regioselectivity, affording four new MLNs analogs. Notably, further genome mining revealed that the orthologs of these three macrolide GT genes showed a regular distribution in the subtilis-and the cereus-clade Bacillus strains; interestingly, bmmGT1 orthologs only occurred in the subtilis-clade Bacillus, and they were also found in the genomes of Streptomyces strains, suggesting their close phylogenetic relationship. These results provide the first significant insight into the important roles of Bacillus macrolide GTs in the biology of the species.
资助项目	Program for New Century Excellent Talents in University[NCET-0900717]
WOS关键词	VANCOMYCIN-RESISTANT ENTEROCOCCI ; AMYLOLIQUEFACIENS FZB42 ; 7-O-SUCCINYL MACROLACTIN ; STAPHYLOCOCCUS-AUREUS ; POLYKETIDE SYNTHASE ; ENZYMATIC-SYNTHESIS ; BIOSYNTHESIS ; PRODUCT ; SUBTILIS ; GLYCOSYLATION
WOS研究方向	Microbiology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000392946900036
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25649]
专题	自然资源部第一海洋研究所
通讯作者	Li, Wenli
作者单位	1.Ocean Univ China, Key Lab Marine Drugs, Minist Educ China, Sch Med & Pharm, Qingdao 266003, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, 6 Xianxialing Rd, Qingdao 266061, SD, Peoples R China 3.Qingdao Univ Sci & Technol, 53 Zhen Zhou Rd, Qingdao 266042, SD, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yang,Qin, Wen,Liu, Quanquan,et al. Genome-wide identification and characterization of macrolide glycosyltransferases from a marine-derived Bacillus strain and their phylogenetic distribution[J]. ENVIRONMENTAL MICROBIOLOGY,2016,18(12):4770-4781.
APA	Liu, Yang.,Qin, Wen.,Liu, Quanquan.,Zhang, Jun.,Li, Huayue.,...&Li, Wenli.(2016).Genome-wide identification and characterization of macrolide glycosyltransferases from a marine-derived Bacillus strain and their phylogenetic distribution.ENVIRONMENTAL MICROBIOLOGY,18(12),4770-4781.
MLA	Liu, Yang,et al."Genome-wide identification and characterization of macrolide glycosyltransferases from a marine-derived Bacillus strain and their phylogenetic distribution".ENVIRONMENTAL MICROBIOLOGY 18.12(2016):4770-4781.