The study of the adductor muscle-shell interface structure in three Mollusc species

doi:10.1007/s13131-016-0878-x

	The study of the adductor muscle-shell interface structure in three Mollusc species
	Zhu Yaoyao 1,2; Sun Chengjun 1,3; Song Yingfei 2; Jiang Fenghua 1; Yin Xiaofei 1; Tang Min 4,5; Ding Haibing 6,7
刊名	ACTA OCEANOLOGICA SINICA
	2016-08
卷号	35 期号:8 页码:57-64
关键词	mollusc shell adductor muscle-shell interface shell protein shell microstructure
ISSN号	0253-505X
DOI	10.1007/s13131-016-0878-x
英文摘要	The adductor muscle scar (AMS) is the fixation point of adductor muscle to the shell. It is an important organic inorganic interface and stress distribution area. Despite recent advances, our understanding of the structure and composition of the AMS remain limited. Here, we report study on the AMS of three bivalves: Mytilus coruscus, Chlamys farreri and Ruditapes philippinarum. Results showed that there were significant differences among their AMS structures. Both M. coruscus and C. farreri were found to have a columnar layer above the nacreous platelet shell structure at the AMS and this layer was more organized in M. coruscus. There was no distinguishable two layer structure in R. philippinarum. Atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FT-IR) results showed that the AMS was much smoother than the nacreous inner shell in all the three species and the AMS had minor different compositions from the nacreous shell layer. SDS-PAGE (sodium dodecyl-sulfate polyacrylamide gel electophoresis) study of the proteins isolated from the interface indicated that there was a 70 kappa Da protein which seemed to be specifically located to the highly organized columnar AMS structure in Mytilus coruscus. Further analysis of this protein showed it contained high level of Asx (Asp+Asn), Glx (Glu+Gln) and Gly. The special structure and composition of the AMS might play important roles in the stability, adhesion and function at this stress distribution site.
资助项目	Qingdao Talents Program[13-CX-20]
WOS关键词	OYSTER-PINCTADA-FUCATA ; CALCIUM-CARBONATE ; PRISMATIC LAYER ; MATRIX PROTEIN ; ORGANIC MATRIX ; PEARL ; SPECTROSCOPY ; MOTHER ; DESIGN ; MUSSEL
WOS研究方向	Oceanography
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000381248900009
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/25337]
专题	自然资源部第一海洋研究所
通讯作者	Ding Haibing
作者单位	1.State Ocean Adm, Inst Oceanog 1, Marine Ecol Ctr, Qingdao 266061, Peoples R China 2.Ocean Univ China, Coll Chem & Chem Engn, Qingdao 266100, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266071, Peoples R China 4.Hainan Univ, Key Lab Adv Mat Trop Isl Resources, Minist Educ, Haikou 570228, Peoples R China 5.Hainan Univ, Coll Mat & Chem Engn, Haikou 570228, Peoples R China 6.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China 7.Ocean Univ China, Qingdao Collaborat Innovat Ctr Marine Sci & Techn, Qingdao 266100, Peoples R China
推荐引用方式 GB/T 7714	Zhu Yaoyao,Sun Chengjun,Song Yingfei,et al. The study of the adductor muscle-shell interface structure in three Mollusc species[J]. ACTA OCEANOLOGICA SINICA,2016,35(8):57-64.
APA	Zhu Yaoyao.,Sun Chengjun.,Song Yingfei.,Jiang Fenghua.,Yin Xiaofei.,...&Ding Haibing.(2016).The study of the adductor muscle-shell interface structure in three Mollusc species.ACTA OCEANOLOGICA SINICA,35(8),57-64.
MLA	Zhu Yaoyao,et al."The study of the adductor muscle-shell interface structure in three Mollusc species".ACTA OCEANOLOGICA SINICA 35.8(2016):57-64.