Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo

doi:10.1016/j.envpol.2019.01.072

	Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo
	Jia, Pan-Pan 1,2; Sun, Tai 1; Junaid, Muhammad 1,2; Yang, Li 1; Ma, Yan-Bo 1; Cui, Zhi-Song 3; Wei, Da-Peng 1; Shi, Hao-Fei 1; Pei, De-Sheng 1
刊名	ENVIRONMENTAL POLLUTION
	2019-04
卷号	247 页码:595-606
关键词	Graphene Graphene oxide Cytotoxicity Anti-bacterial property Zebrafish
ISSN号	0269-7491
DOI	10.1016/j.envpol.2019.01.072
英文摘要	Graphene family nanomaterials (GFNs) have attracted significant attention due to their unique characteristics and applications in the fields of biomedicine and nanotechnology. However, previous studies highlighted the in vitro and in vivo toxicity of GFNs with size and oxidation state differences are still elusive. Therefore, we prepared graphene (G) and graphene oxide (GO) of three different sizes (S-small, M-medium, and L-large), and characterized them using multiple surface-sensitive analytical techniques. In vitro assays using HEK 293T cells revealed that the small and large sizes of G and GO significantly reduced the cell viability and increased DNA damage, accompanying with activated reactive oxygen species (ROS) generation and induced various expressions of associated critical genetic markers. Moreover, the bacterial assays highlighted that G and GO caused strong acute toxicity on Tox2 bacteria. Effects of G were higher than GO and showed size dependent effect: L>M>S, while the medium size of GO induced mild genetic toxicity on RecA bacteria. In vivo assays revealed that exposure to G and GO caused the developmental toxicity, induced ROS generation, and activated related pathways (specifically GO) in zebrafish. Taken together, G showed stronger ability to decrease the survival rate and induce the acute toxicity, while GO showed obvious toxicity in terms of DNA damages, ROS generation, and abnormal gene expressions. Our findings highlighted that G and GO differentially induced toxicity based on their varying physical characteristics, especially sizes and oxidation state, and exposure concentrations and sensitivity of the employed in vitro and in vivo models. In short, this study provided deep insights on the negative effects of GFNs exposure. (C) 2019 Elsevier Ltd. All rights reserved.
资助项目	Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20004] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2014yykfC20002] ; Key Application and Development Program of Chongqing Science and Technology Commission[cstc2016jcyjA0314]
WOS关键词	GROWTH-FACTOR RECEPTOR ; CARBON NANOTUBES ; OXIDATIVE STRESS ; DNA-DAMAGE ; BIOMEDICAL APPLICATIONS ; ZEBRAFISH ; TOXICITY ; CYTOTOXICITY ; REPAIR ; NANOSHEETS
WOS研究方向	Environmental Sciences & Ecology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000460844800065
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/24811]
专题	自然资源部第一海洋研究所
通讯作者	Pei, De-Sheng
作者单位	1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.State Ocean Adm, Marine Ecol Res Ctr, Inst Oceanog 1, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Jia, Pan-Pan,Sun, Tai,Junaid, Muhammad,et al. Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo[J]. ENVIRONMENTAL POLLUTION,2019,247:595-606.
APA	Jia, Pan-Pan.,Sun, Tai.,Junaid, Muhammad.,Yang, Li.,Ma, Yan-Bo.,...&Pei, De-Sheng.(2019).Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo.ENVIRONMENTAL POLLUTION,247,595-606.
MLA	Jia, Pan-Pan,et al."Nanotoxicity of different sizes of graphene (G) and graphene oxide (GO) in vitro and in vivo".ENVIRONMENTAL POLLUTION 247(2019):595-606.