Bacterial community pattern along the sediment seafloor of the Arctic fjorden (Kongsfjorden, Svalbard)

doi:10.1007/s10482-019-01245-z

	Bacterial community pattern along the sediment seafloor of the Arctic fjorden (Kongsfjorden, Svalbard)
	Fang, Xiao-Mei 1,2; Zhang, Tao 1,2; Li, Jun 1,2; Wang, Neng-Fei 3; Wang, Zhen 4; Yu, Li-Yan 1,2
刊名	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
	2019-08
卷号	112 期号:8 页码:1121-1136
关键词	Bacterial community pattern High Arctic Kongsfjorden Illumina MiSeq sequencing Marine sediments
ISSN号	0003-6072
DOI	10.1007/s10482-019-01245-z
英文摘要	The Arctic region has been the focus of increasing attention as an ecosystem that is highly sensitive to changes associated with global warming. Although it was assumed to be vulnerable to changes in climate, a limited number of studies have been conducted on the surface sediment bacteria of Arctic fjorden. This study assessed the diversity and distribution pattern of bacterial communities in eight marine sediments along the seafloor in a high Arctic fjorden (Kongsfjorden, Svalbard). A total of 822 operational taxonomic units (OTUs) were identified by Illumina MiSeq sequencing, targeting the V3-V4 hypervariable regions of the 16S rRNA gene. In these surface marine sediments, more than half of the sequences belonged to the phylum Proteobacteria, followed by Bacteroidetes, Verrucomicrobia, Actinobacteria, Chloroflexi and Lentisphaerae. The bacterial genera Marinicella, Desulfobulbus, Lutimonas, Sulfurovum and clade SEEP-SRB4 were dominant in all samples. Analysis of similarity indicated that bacterial communities were significantly different among the inner, central and outer basins (r(2)=0.5, P=0.03<0.05). Canonical correspondence analysis and permutation tests revealed that location depth (r(2)=0.87, P<0.01), temperature (r(2)=0.88, P<0.01) and salinity (r(2)=0.88, P<0.05) were the most significant factors that correlated with the bacterial communities in the sediments. 28 differentially abundant taxonomic clades in the inner and outer basin with an LDA score higher than 2.0 were found by the LEfSe method. The Spearman correlation heat map revealed different degrees of correlation between most major OTUs and environmental factors, while some clades have an inverse correlation with environmental factors. The spatial patterns of bacterial communities along the Kongsfjorden may offer insight into the ecological responses of prokaryotes to climate change in the Arctic ecosystem, which makes it necessary to continue with monitoring.
资助项目	Beijing Science and Technology Projects[Z141102004414065]
WOS关键词	MARINE-SEDIMENTS ; ORGANIC-MATTER ; DIVERSITY ; SENSITIVITY ; PROTISTAN ; OCEAN
WOS研究方向	Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000475517500001
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/24583]
专题	自然资源部第一海洋研究所
通讯作者	Yu, Li-Yan
作者单位	1.Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China 2.Peking Union Med Coll, Beijing 100050, Peoples R China 3.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Shandong, Peoples R China 4.Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Fang, Xiao-Mei,Zhang, Tao,Li, Jun,et al. Bacterial community pattern along the sediment seafloor of the Arctic fjorden (Kongsfjorden, Svalbard)[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2019,112(8):1121-1136.
APA	Fang, Xiao-Mei,Zhang, Tao,Li, Jun,Wang, Neng-Fei,Wang, Zhen,&Yu, Li-Yan.(2019).Bacterial community pattern along the sediment seafloor of the Arctic fjorden (Kongsfjorden, Svalbard).ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,112(8),1121-1136.
MLA	Fang, Xiao-Mei,et al."Bacterial community pattern along the sediment seafloor of the Arctic fjorden (Kongsfjorden, Svalbard)".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 112.8(2019):1121-1136.