Magnetite-Containing Spherical Silica Nanoparticles for Biocatalysis and Bioseparations

doi:10.1021/ac034920m

	Magnetite-Containing Spherical Silica Nanoparticles for Biocatalysis and Bioseparations
	Yang, Huang-Hao 1; Zhang, Shu-Qiong 2; Chen, Xiao-Lan 2; Zhuang, Zhi-Xia 1,2; Xu, Jin-Gou 2; Wang, Xiao-Ru 1,2
刊名	Analytical Chemistry
	2004-03-01
卷号	76 期号:5 页码:1316-1321
ISSN号	00032700
DOI	10.1021/ac034920m
英文摘要	The simultaneous entrapment of biological macromolecules and nanostructured silica-coated magnetite in sol-gel materials using a reverse-micelle technique leads to a bioactive, mechanically stable, nanometer-sized, and magnetically separable particles. These spherical particles have a typical diameter of 53 ± 4 nm, a large surface area of 330 m2/g, an average pore diameter of 1.5 nm, a total pore volume of 1.427 cm3/g and a saturated magnetization (MS) of 3.2 emu/g. Peroxidase entrapped in these particles shows Michaelis-Mentan kinetics and high activity. The catalytic reaction will take place immediately after adding these particles to the reaction solution. These enzyme entrapping particles catalysts can be easily separated from the reaction mixture by simply using an external magnetic field. Experiments have proved that these catalysts have a long-term stability toward temperature and pH change, as compared to free enzyme molecules. To further prove the application of this novel magnetic biomaterial in analytical chemistry, a magnetic-separation immunoassay system was also developed for the quantitative determination of gentamicin. The calibration for gentamicin has a working range of 200-4000 ng/mL, with a detection limit of 160 ng/mL, which is close to that of the fluorescent polarization immunoassay (FPIA) using the same reactants.
语种	英语
出版者	American Chemical Society
内容类型	期刊论文
源URL	[http://ir.fio.com.cn:8080/handle/2SI8HI0U/6904]
专题	自然资源部第一海洋研究所
作者单位	1.Mat. Transport and Transformation, First Institute of Oceanography, SOA, Qingdao, 266061, China; 2.Key Lab. of Analyt. Sciences of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, China
推荐引用方式 GB/T 7714	Yang, Huang-Hao,Zhang, Shu-Qiong,Chen, Xiao-Lan,et al. Magnetite-Containing Spherical Silica Nanoparticles for Biocatalysis and Bioseparations[J]. Analytical Chemistry,2004,76(5):1316-1321.
APA	Yang, Huang-Hao,Zhang, Shu-Qiong,Chen, Xiao-Lan,Zhuang, Zhi-Xia,Xu, Jin-Gou,&Wang, Xiao-Ru.(2004).Magnetite-Containing Spherical Silica Nanoparticles for Biocatalysis and Bioseparations.Analytical Chemistry,76(5),1316-1321.
MLA	Yang, Huang-Hao,et al."Magnetite-Containing Spherical Silica Nanoparticles for Biocatalysis and Bioseparations".Analytical Chemistry 76.5(2004):1316-1321.