Manganese/polymetallic nodules: Micro-structural characterization of exolithobiontic- and endolithobiontic microbial biofilms by scanning electron microscopy

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	Manganese/polymetallic nodules: Micro-structural characterization of exolithobiontic- and endolithobiontic microbial biofilms by scanning electron microscopy
	Wang, XH (Wang, Xiaohong); Schroder, HC (Schroeder, Heinz C.); Wiens, M (Wiens, Matthias); Schlossmacher, U (Schlossmacher, Ute); Muller, WEG (Mueller, Werner E. G.)
刊名	MICRON
	2009
卷号	40 期号:3 页码:350-358
关键词	Manganese nodules Polymetallic nodules Biogenic mineralization Lithoautotrophic microorganisms Biofilm Clarion-Clipperton zone
ISSN号	0968-4328
英文摘要	Polymetallic/ferromanganese nodules (Mn-nodules) provide a rich source for manganese. It is not yet known if the nodules have a biogenic or an abiogenic origin. Here we applied the technique of high-resolution scanning electron microscopy, in combination with energy dispersive X-ray spectroscopical (EDX) analysis, to trace the existence of microbial biofilms. Two spatially separated assemblies exist, the exolithobiontic- and endolithobiontic colonizations. The exolithobiontic colonization is seen in the micro-canals, which traverse the outer surface layer of the nodules and are formed by elongated filamentous organisms, which show no signs of mineralization. In the center of the nodules three types of endolithobiontic microbial biofilms exist first, cone-like microorganisms forming biofilms, second stone/pillar-like microorganisms and finally paving stone-like, hexagonal microorganisms. All are covered by brick-like mineral deposits. By EDX analysis we could measured the highest relative level of carbon (C) with respect to manganese (Mn) and sodium on the microorganisms. Our data are in perfect agreement with the assumption that the Mn deposits in the nodules are of biogenic origin. In a first approach, DNA from microorganisms from the interior of those Mn-nodules was isolated by PCR, and sequenced with respect to the 16S ribosomal RNA gene. Sequence comparison revealed that the sequence from the Mn-nodule, studied here, shares highest similarity to a bacterium living in soil, rich in iron and manganese. We propose that the microorganisms form a biofilm within the nodules onto which Mn is deposited due to an oxidation from Mn(II) to Mn(III)/Mn(IV). (C) 2008 Elsevier Ltd. All rights reserved.
学科主题	岩矿鉴定与测试
WOS关键词	MANGANESE NODULES ; SEA-FLOOR ; SILICATEIN ; BACTERIA ; COMMUNITIES ; DIVERSITY ; OXIDATION ; ORIGIN ; OCEAN ; LIFE
WOS研究方向	Microscopy
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000263780400009
内容类型	期刊论文
源URL	[http://119.78.100.197/handle/2HKVOGP0/38902]
专题	中国地质调查局
作者单位	Inst Physiol Chem, Angew Mol Biol Abt, D-55099 Mainz, Germany
推荐引用方式 GB/T 7714	Wang, XH ,Schroder, HC ,Wiens, M ,et al. Manganese/polymetallic nodules: Micro-structural characterization of exolithobiontic- and endolithobiontic microbial biofilms by scanning electron microscopy[J]. MICRON,2009,40(3):350-358.
APA	Wang, XH ,Schroder, HC ,Wiens, M ,Schlossmacher, U ,&Muller, WEG .(2009).Manganese/polymetallic nodules: Micro-structural characterization of exolithobiontic- and endolithobiontic microbial biofilms by scanning electron microscopy.MICRON,40(3),350-358.
MLA	Wang, XH ,et al."Manganese/polymetallic nodules: Micro-structural characterization of exolithobiontic- and endolithobiontic microbial biofilms by scanning electron microscopy".MICRON 40.3(2009):350-358.