Biocompatibility of iron carbide and detection of metals ions signaling   proteomic analysis via HPLC/ESI-Orbitrap

doi:10.1007/s12274-016-1375-4

CORC > 北京大学 > 工学院

	Biocompatibility of iron carbide and detection of metals ions signaling proteomic analysis via HPLC/ESI-Orbitrap
	Hasan, Murtaza ; Yang, Wenlong ; Ju, Yanmin ; Chu, Xin ; Wang, Yun ; Deng, Yulin ; Mahmood, Nasir ; Hou, Yanglong
刊名	NANO RESEARCH
	2017
关键词	biocompatibility O-18 labeling magnetic nanoparticles proteomic analysis SIMULATED MICROGRAVITY RAT HYPOTHALAMUS NANOPARTICLES STABILITY PROTEINS CA2+-CALMODULIN TRANSKETOLASE CALMODULIN EXPRESSION PROVIDE
DOI	10.1007/s12274-016-1375-4
英文摘要	Recently, magnetic nanoparticles (NPs) have been extensively used in food industry and biomedical treatments. However, the biocompatibility mechanism on expression proteomics, before consideration of magnetic NPs for clinical application, has not yet been fully elucidated. Therefore, this study was undertaken to identify potential biomarkers of metal ion signaling proteins in human cervical cancer cell line (HeLa) cells. Here, we report the in vitro investigations of the cell cycle response and significant changes in protein abundance of HeLa cells when exposed to self-tailored hydrophilic Fe2C NPs. The comparative proteomic approach based on O-18 labeling coupled with high performance liquid chromatography/ electrospray ionization with ion trap mass analyzer (HPLC/ESI-Orbitrap) was applied, and 394 proteins were identified. There were 46 significantly differentiated proteins based on the specific metal ion signaling response. Among them, 60S ribosomal protein L37a, serine/arginine-rich splicing factor 7, calmodulin, and calumenin were downregulated, whereas transketolase was overexpressed. Functional interaction network of Fe2C-regulated proteins was successfully created by the STRING algorithm to show the strong interactions between proteins. This work will not only help to understand the molecular mechanism of metal ion signaling proteins that can potentially be used to develop therapeutic protocols for diagnosis of diseases but also give direction for tailoring biocompatible magnetic NPs.; Research Fellowship for International Young Scientists of the National Natural Science Foundation of China [51550110502, 51450110437]; National Natural Science Foundation of China [81421004, 51672010, 51590882]; NSFC/RGC Joint Research Scheme [51361165201]; Beijing National Laboratory for Molecular Science; SCI(E); 中国科学引文数据库(CSCD); ARTICLE; 6; 1912-1923; 10
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/473231]
专题	工学院
推荐引用方式 GB/T 7714	Hasan, Murtaza,Yang, Wenlong,Ju, Yanmin,et al. Biocompatibility of iron carbide and detection of metals ions signaling proteomic analysis via HPLC/ESI-Orbitrap[J]. NANO RESEARCH,2017.
APA	Hasan, Murtaza.,Yang, Wenlong.,Ju, Yanmin.,Chu, Xin.,Wang, Yun.,...&Hou, Yanglong.(2017).Biocompatibility of iron carbide and detection of metals ions signaling proteomic analysis via HPLC/ESI-Orbitrap.NANO RESEARCH.
MLA	Hasan, Murtaza,et al."Biocompatibility of iron carbide and detection of metals ions signaling proteomic analysis via HPLC/ESI-Orbitrap".NANO RESEARCH (2017).