A Frequency Domain Reconstruction of SIM Image using four Raw Images

doi:10.1117/12.2246242

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	A Frequency Domain Reconstruction of SIM Image using four Raw Images
	Lal, Amit ; Huang XiaoShuai ; Peng Xi
	2017
关键词	Structured illumination SIM super-resolution optical transfer function STRUCTURED-ILLUMINATION MICROSCOPY RESOLUTION
DOI	10.1117/12.2246242
英文摘要	This work is a result of an exploration in response to the following observation: though reconstruction methodology of SIM image since its origin is carried out in frequency domain, recent works in this field, specially when it comes to SIM reconstruction using lesser than 9 raw images, have ventured into reconstruction methodologies that operate directly in the spatial domain. This work formulates and demonstrates a frequency domain reconstruction of SIM image using four raw images - one wide-field image and three SIM images. One of the chief feature of the presented reconstruction algorithm is that it employs the standard 'tools and tricks' used by the conventional 9-frame SIM reconstruction algorithm. Results indicate that the presented reconstruction algorithm provides high resolution reconstructions successfully as long as the noise level in the raw images is lower than 10%. For higher level of noise, the reconstruction result shows little resolution enhancement.; National Instrument Development Special Program [2013YQ03065102]; National Natural Science Foundation of China [61178076, 61475010, 31327901]; CPCI-S(ISTP); 0024
会议录	Conference on Optics in Health Care and Biomedical Optics VII
语种	英语
内容类型	会议论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/469954]
专题	工学院
推荐引用方式 GB/T 7714	Lal, Amit,Huang XiaoShuai,Peng Xi. A Frequency Domain Reconstruction of SIM Image using four Raw Images[C]. 见:.