Physically entrapped gelatin in polyethylene glycol scaffolds for   three-dimensional chondrocyte culture

doi:10.1177/0883911516633893

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	Physically entrapped gelatin in polyethylene glycol scaffolds for three-dimensional chondrocyte culture
	Zhang, Jingjing ; Mujeeb, Ayeesha ; Feng, Junxia ; Li, Yijiang ; Du, Yanan ; Lin, Jianhao ; Ge, Zigang
刊名	JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS
	2016
关键词	Cell adhesion gelatin physical entrapment macroporous hydrogels chondrocyte culture TISSUE ENGINEERING APPLICATIONS MESENCHYMAL STEM-CELLS POLY(L-LACTIDE-CO-EPSILON-CAPROLACTONE) SCAFFOLD DIACRYLATE HYDROGELS SIGNAL EXPRESSION CHITOSAN ADHESION 3D DIFFERENTIATION ENCAPSULATION
DOI	10.1177/0883911516633893
英文摘要	Developing tissue-engineered constructs for clinical use must satisfy the fundamental biologic parameters of biocompatibility, cell adhesiveness, and biodegradability. Physical entrapment of bioactive agents into synthetic polymers, as three-dimensional scaffolds, holds great promise for cell culture applications. Here, in an attempt to elucidate the effects of physical interlocking of natural and synthetic gel networks on cell responses within three-dimensional microenvironments, gelatin (of different concentrations) was physically incorporated into macroporous polyethylene glycol (PEG) hydrogels to fabricate PEG-GEL1 (10:1, PEG:gelatin) and PEG-GEL5 (10:5, PEG:gelatin). The effect of the physically entrapped gelatin on primary chondrocytes was investigated in relation to cell distribution, morphology and viability, proliferation, gene expression, and extracellular matrix accumulation in vitro. Our findings have shown successful incorporation of two different concentrations of gelatin into polyethylene glycol macroporous hydrogels through physical mixing. These physical blends not only enhanced chondrocyte adhesion and proliferation but also boosted gene expression of collagen II and aggrecan after 14days in culture. Although results demonstrated that gelatin levels dropped sharply in PEG-GEL1 and PEG-GEL5 in the first 7days, however evidently, after days 14 and 21 gelatin levels in both groups remained substantially unchanged and in turn enhanced glycosaminoglycan formation in vitro. Thus, the modification of polyethylene-glycol-based scaffolds with physically entrapped gelatin may be sufficient for dictating three-dimensional microenvironments for chondrocyte cultures.; National Basic Research Program of China grant (973 Program) [2012CB619100]; National Natural Science Foundation of China [81471800, 81271722]; SCI(E); EI; ARTICLE; gez@pku.edu.cn; 5; 513-530; 31
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/448026]
专题	工学院
推荐引用方式 GB/T 7714	Zhang, Jingjing,Mujeeb, Ayeesha,Feng, Junxia,et al. Physically entrapped gelatin in polyethylene glycol scaffolds for three-dimensional chondrocyte culture[J]. JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS,2016.
APA	Zhang, Jingjing.,Mujeeb, Ayeesha.,Feng, Junxia.,Li, Yijiang.,Du, Yanan.,...&Ge, Zigang.(2016).Physically entrapped gelatin in polyethylene glycol scaffolds for three-dimensional chondrocyte culture.JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS.
MLA	Zhang, Jingjing,et al."Physically entrapped gelatin in polyethylene glycol scaffolds for three-dimensional chondrocyte culture".JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS (2016).