Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells In Vitro and In Vivo | |
Liu, Yunsong ; Chen, Tong ; Du, Feng ; Gu, Ming ; Zhang, Ping ; Zhang, Xiao ; Liu, Jianzhang ; Lv, Longwei ; Xiong, Chunyang ; Zhou, Yongsheng | |
刊名 | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY |
2016 | |
关键词 | Graphene Mesenchymal Stem Cells Osteogenic Differentiation Epigenetics BONE REGENERATION STROMAL CELLS PROPERTY RELATIONSHIP CELLULAR-RESPONSE TIO2 NANOTUBES LARGE-AREA OXIDE FILMS OSTEOBLASTS PROTEIN |
DOI | 10.1166/jbn.2016.2254 |
英文摘要 | In recent years, although several studies have demonstrated the potential of graphene-coated substrates in promoting attachment, proliferation and differentiation of osteoblasts and mesenchymal stem cells (MSCs), the effects of single-layer graphene on the osteogenic differentiation of human MSCs (hMSCs) remains unclear, especially in vivo. In this study, we transferred chemical vapor deposition (CVD) grown single-layer graphene to glass slides and observed its effects on adhesion, proliferation and osteogenic differentiation of human adipose-derived stem cells (hASCs) and human bone marrow mesenchymal stem cells (hBMMSCs) in vitro. Then, in vivo, we incubated hASCs and hBMMSCs on single layer graphene-coated smooth titanium (Ti) disks before implanting them into the back subcutaneous area of nude mice. We found that single-layer graphene accelerated cell adhesion to the substrate without influencing cell proliferation of hMSCs. Moreover, we present the first study that explores the epigenetic role of single-layer graphene in determining stem cell fate. By utilizing epigenetic approaches, we reveal that single-layer graphene promotes osteogenic differentiation of hMSCs both in vitro and in vivo, potentially by upregulating methylation of H3K4 at the promoter regions of osteogenesis-associated genes. Overall, our results highlight the potential of this material in implants and injured tissues in clinical applications.; Ministry of Science and Technology of China [2013CB933702]; National Natural Science Foundation of China [81470769, 11272015]; Beijing Natural Science Foundation [7153178]; Program for New Century Excellent Talents in University from Ministry of Education of China [NCET-11-0026]; Peking University 985 Program [2012]; SCI(E); EI; ARTICLE; cyxiong@pku.edu.cn; kqzhouysh@hsc.pku.edu.cn; 6; 1270-1284; 12 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/437158] |
专题 | 工学院 |
推荐引用方式 GB/T 7714 | Liu, Yunsong,Chen, Tong,Du, Feng,et al. Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells In Vitro and In Vivo[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2016. |
APA | Liu, Yunsong.,Chen, Tong.,Du, Feng.,Gu, Ming.,Zhang, Ping.,...&Zhou, Yongsheng.(2016).Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells In Vitro and In Vivo.JOURNAL OF BIOMEDICAL NANOTECHNOLOGY. |
MLA | Liu, Yunsong,et al."Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells In Vitro and In Vivo".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY (2016). |
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