Crude oil as a microbial seed bank with unexpected functional potentials

doi:10.1038/srep16057

CORC > 北京大学 > 工学院

	Crude oil as a microbial seed bank with unexpected functional potentials
	Cai, Man ; Nie, Yong ; Chi, Chang-Qiao ; Tang, Yue-Qin ; Li, Yan ; Wang, Xing-Biao ; Liu, Ze-Shen ; Yang, Yunfeng ; Zhou, Jizhong ; Wu, Xiao-Lei
刊名	SCIENTIFIC REPORTS
	2015
关键词	TEMPERATURE PETROLEUM RESERVOIR PSEUDOMONAS-AERUGINOSA PHYLOGENETIC DIVERSITY BACTERIAL COMMUNITIES WATER FIELD BIODEGRADATION RECOVERY BIOREMEDIATION IDENTIFICATION
DOI	10.1038/srep16057
英文摘要	It was widely believed that oil is a harsh habitat for microbes because of its high toxicity and hydrophobicity. However, accumulating evidence has revealed the presence of live microbes in crude oil. Therefore, it's of value to conduct an in-depth investigation on microbial communities in crude oil. To this end, microorganisms in oil and water phases were collected from four oil-well production mixtures in Qinghai Oilfield, China, and analyzed for their taxonomic and functional compositions via pyrosequencing and GeoChip, respectively. Hierarchical clustering of 16S rRNA gene sequences and functional genes clearly separated crude oil and water phases, suggestive of distinct taxonomic and functional gene compositions between crude oil and water phases. Unexpectedly, Pseudomonas dominated oil phase where diverse functional gene groups were identified, which significantly differed from those in the corresponding water phases. Meanwhile, most functional genes were significantly more abundant in oil phase, which was consistent with their important roles in facilitating survival of their host organisms in crude oil. These findings provide strong evidence that crude oil could be a "seed bank" of functional microorganisms with rich functional potentials. This offers novel insights for industrial applications of microbial-enhanced oil recovery and bioremediation of petroleum-polluted environments.; National Natural Science Foundation of China [31225001]; National High Technology Research and Development Program [2012AA02A703, 2014AA021505]; SCI(E); PubMed; ARTICLE; xiaolei_wu@pku.edu.cn; 16057; 5
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/415336]
专题	工学院
推荐引用方式 GB/T 7714	Cai, Man,Nie, Yong,Chi, Chang-Qiao,et al. Crude oil as a microbial seed bank with unexpected functional potentials[J]. SCIENTIFIC REPORTS,2015.
APA	Cai, Man.,Nie, Yong.,Chi, Chang-Qiao.,Tang, Yue-Qin.,Li, Yan.,...&Wu, Xiao-Lei.(2015).Crude oil as a microbial seed bank with unexpected functional potentials.SCIENTIFIC REPORTS.
MLA	Cai, Man,et al."Crude oil as a microbial seed bank with unexpected functional potentials".SCIENTIFIC REPORTS (2015).