Entropy-Based Model for MiRNA Isoform Analysis

doi:10.1371/journal.pone.0118856

CORC > 北京大学 > 工学院

	Entropy-Based Model for MiRNA Isoform Analysis
	Wang, Shengqin ; Tu, Jing ; Wang, Lei ; Lu, Zuhong
刊名	plos one
	2015
关键词	LARGE GENE LISTS MICRORNAS ISOMIRS COMPLEXITY EXPRESSION PATTERNS SEQUENCE CANCER IDENTIFICATION REPERTOIRE
DOI	10.1371/journal.pone.0118856
英文摘要	MiRNAs have been widely studied due to their important post-transcriptional regulatory roles in gene expression. Many reports have demonstrated the evidence of miRNA isoform products (isomiRs) in high-throughput small RNA sequencing data. However, the biological function involved in these molecules is still not well investigated. Here, we developed a Shannon entropy-based model to estimate isomiR expression profiles of high-throughput small RNA sequencing data extracted from miRBase webserver. By using the Kolmogorov-Smirnov statistical test (KS test), we demonstrated that the 5p and 3p miRNAs present more variants than the single arm miRNAs. We also found that the isomiR variant, except the 3' isomiR variant, is strongly correlated with Minimum Free Energy (MFE) of pre-miRNA, suggesting the intrinsic feature of pre-miRNA should be one of the important factors for the miRNA regulation. The functional enrichment analysis showed that the miRNAs with high variation, particularly the 5' end variation, are enriched in a set of critical functions, supporting these molecules should not be randomly produced. Our results provide a probabilistic framework for miRNA isoforms analysis, and give functional insights into pre-miRNA processing.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000352138500052&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 2; ARTICLE; zhlu@seu.edu.cn; 3; e0118856; 10
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/341391]
专题	工学院
推荐引用方式 GB/T 7714	Wang, Shengqin,Tu, Jing,Wang, Lei,et al. Entropy-Based Model for MiRNA Isoform Analysis[J]. plos one,2015.
APA	Wang, Shengqin,Tu, Jing,Wang, Lei,&Lu, Zuhong.(2015).Entropy-Based Model for MiRNA Isoform Analysis.plos one.
MLA	Wang, Shengqin,et al."Entropy-Based Model for MiRNA Isoform Analysis".plos one (2015).