An atmospheric-pressure cold plasma leads to apoptosis in Saccharomyces   cerevisiae by accumulating intracellular reactive oxygen species and   calcium

doi:10.1088/0022-3727/46/28/285401

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	An atmospheric-pressure cold plasma leads to apoptosis in Saccharomyces cerevisiae by accumulating intracellular reactive oxygen species and calcium
	Ma, R. N. ; Feng, H. Q. ; Liang, Y. D. ; Zhang, Q. ; Tian, Y. ; Su, B. ; Zhang, J. ; Fang, J.
刊名	journal of physics d applied physics
	2013
关键词	OXIDATIVE STRESS AIR PLASMA YEAST INACTIVATION MEDICINE BIOFILMS BACTERIA THERAPY SPARKS
DOI	10.1088/0022-3727/46/28/285401
英文摘要	A non-thermal plasma is known to induce apoptosis of various cells but the mechanism is not yet clear. A eukaryotic model organism Saccharomyces cerevisiae was used to investigate the cellular and biochemical regulations of cell apoptosis and cell cycle after an atmospheric-pressure cold plasma treatment. More importantly, intracellular calcium (Ca2+) was first involved in monitoring the process of plasma-induced apoptosis in this study. We analysed the cell apoptosis and cell cycle by flow cytometry and observed the changes in intracellular reactive oxygen species (ROS) and Ca2+ concentration, cell mitochondrial membrane potential (Delta psi(m)) as well as nuclear DNA morphology via fluorescence staining assay. All experimental results indicated that plasma-generated ROS leads to the accumulation of intracellular ROS and Ca2+ that ultimately contribute to apoptosis associated with cell cycle arrest at G1 phase through depolarization of Delta psi(m) and fragmenting nuclear DNA. This work provides a novel insight into the physical and biological mechanism of apoptosis induced by a plasma which could benefit for promoting the development of plasmas applied to cancer therapy.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000320928700018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Physics, Applied; SCI(E); EI; 15; ARTICLE; 28; 46
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/327109]
专题	工学院
推荐引用方式 GB/T 7714	Ma, R. N.,Feng, H. Q.,Liang, Y. D.,et al. An atmospheric-pressure cold plasma leads to apoptosis in Saccharomyces cerevisiae by accumulating intracellular reactive oxygen species and calcium[J]. journal of physics d applied physics,2013.
APA	Ma, R. N..,Feng, H. Q..,Liang, Y. D..,Zhang, Q..,Tian, Y..,...&Fang, J..(2013).An atmospheric-pressure cold plasma leads to apoptosis in Saccharomyces cerevisiae by accumulating intracellular reactive oxygen species and calcium.journal of physics d applied physics.
MLA	Ma, R. N.,et al."An atmospheric-pressure cold plasma leads to apoptosis in Saccharomyces cerevisiae by accumulating intracellular reactive oxygen species and calcium".journal of physics d applied physics (2013).