Engineered TAL Effector modulators for the large-scale gain-of-function   screening

doi:10.1093/nar/gku535

CORC > 北京大学 > 工学院

	Engineered TAL Effector modulators for the large-scale gain-of-function screening
	Zhang, Hanshuo ; Li, Juan ; Hou, Sha ; Wang, Gancheng ; Jiang, Mingjun ; Sun, Changhong ; Hu, Xiongbing ; Zhuang, Fengfeng ; Dai, Zhifei ; Dai, Junbiao ; Xi, Jianzhong Jeff
刊名	核酸研究
	2014
关键词	TRANSCRIPTION FACTORS GENOME BROWSER III EFFECTORS MOUSE MODEL EXPRESSION PROTEINS UCSC
DOI	10.1093/nar/gku535
英文摘要	Recent effective use of TAL Effectors (TALEs) has provided an important approach to the design and synthesis of sequence-specific DNA-binding proteins. However, it is still a challenging task to design and manufacture effective TALE modulators because of the limited knowledge of TALE-DNA interactions. Here we synthesized more than 200 TALE modulators and identified two determining factors of transcription activity in vivo: chromatin accessibility and the distance from the transcription start site. The implementation of these modulators in a gain-of-function screen was successfully demonstrated for four cell lines in migration/invasion assays and thus has broad relevance in this field. Furthermore, a novel TALE-TALE modulator was developed to transcriptionally inhibit target genes. Together, these findings underscore the huge potential of these TALE modulators in the study of gene function, reprogramming of cellular behaviors, and even clinical investigation.; Biochemistry & Molecular Biology; SCI(E); PubMed; 0; ARTICLE; jbdai@biomed.tsinghua.edu.cn; jzxi@pku.edu.cn; 14; e114; 42
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/315625]
专题	工学院
推荐引用方式 GB/T 7714	Zhang, Hanshuo,Li, Juan,Hou, Sha,et al. Engineered TAL Effector modulators for the large-scale gain-of-function screening[J]. 核酸研究,2014.
APA	Zhang, Hanshuo.,Li, Juan.,Hou, Sha.,Wang, Gancheng.,Jiang, Mingjun.,...&Xi, Jianzhong Jeff.(2014).Engineered TAL Effector modulators for the large-scale gain-of-function screening.核酸研究.
MLA	Zhang, Hanshuo,et al."Engineered TAL Effector modulators for the large-scale gain-of-function screening".核酸研究 (2014).