炭气凝胶纳米颗粒固定葡萄糖氧化酶的直接电化学

doi:10.3866/PKU.WHXB201209201

CORC > 北京大学 > 工学院

	炭气凝胶纳米颗粒固定葡萄糖氧化酶的直接电化学; Direct Electrochemistry and Immobilization of Glucose Oxidase on Nano-Carbon Aerogels
	于志辉 ; 宿婷婷 ; 任翠华 ; 李钒 ; 夏定国 ; *程水源
刊名	物理化学学报
	2012
关键词	炭气凝胶葡萄糖氧化酶直接电化学 Carbon aerogel Glucose oxidase Direct electrochemistry
DOI	10.3866/PKU.WHXB201209201
英文摘要	利用间苯二酚和甲醛在碱性环境下制备炭气凝胶(CA),通过扫描电镜(SEM)、透射电镜(TEM)、比表面积测试Brunauer-Emmett-Tel er (BET)等方法分析载体的形貌结构;以CA为载体通过吸附法固定葡萄糖氧化酶(GOD)并修饰玻碳(GC)电极,得到GOD/CA/GC电极.在0.1 mol·L-1磷酸盐缓冲溶液中,利用循环伏安法研究了GOD/CA/GC电极的直接电化学行为和对葡萄糖的催化性能.结果表明,以CA为载体可以很好地固定GOD并保持其生物活性,在无任何电子媒介体存在时, GOD在电极上实现了直接电子转移, GOD/CA/GC电极对葡萄糖具有很好的电催化性能.; Nano-carbon aerogels (CA) were prepared by the reaction between resorcinol and formaldehyde in alkaline conditions. The morphology and microstructure of the CA was investigated by scanning electron microscopy and transmission electron microscopy. The specific area and the pore size distribution were calculated from the N-2 adsorption-desorption isotherm. Glucose oxidase (GOD)/CA/glassy carbon (GC) electrodes were prepared by immobilizing GOD on the surface of nano-carbon aerogels. The electrocatalytic properties of GOD/CA/GC electrodes were characterized by cyclic voltammetry in phosphate buffer medium (0.1 mol.L-1). The results showed that GOD can be immobilized on the nano-carbon aerogels, and that the biological activation of GOD was retained. The GOD/CA/GC electrode exhibits good direct electrochemical and electro-catalytic performance without electron mediators.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000311938700015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 4; 12; 2867-2873; 28
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/229209]
专题	工学院
推荐引用方式 GB/T 7714	于志辉,宿婷婷,任翠华,等. 炭气凝胶纳米颗粒固定葡萄糖氧化酶的直接电化学, Direct Electrochemistry and Immobilization of Glucose Oxidase on Nano-Carbon Aerogels[J]. 物理化学学报,2012.
APA	于志辉,宿婷婷,任翠华,李钒,夏定国,&*程水源.(2012).炭气凝胶纳米颗粒固定葡萄糖氧化酶的直接电化学.物理化学学报.
MLA	于志辉,et al."炭气凝胶纳米颗粒固定葡萄糖氧化酶的直接电化学".物理化学学报 (2012).