Synthesis and fluorescence study of a pyrene-functionalized   poly(4-vinylpyridine) quaternary ammonium for detection of DNA   hybridization

doi:10.1016/j.polymer.2012.12.080

CORC > 北京大学 > 工学院

	Synthesis and fluorescence study of a pyrene-functionalized poly(4-vinylpyridine) quaternary ammonium for detection of DNA hybridization
	Zhang, Ruichen ; Yang, Lingye ; Zhao, Min ; Dong, Jie ; Dong, Haifeng ; Wen, Yongqiang ; Zhan, Xiaowei ; Yang, Huai ; Wang, Guojie
刊名	polymer
	2013
关键词	Fluorescence Polyelectrolyte Probe SOLUBLE CONJUGATED POLYMERS DOUBLE-STRANDED DNA ENERGY-TRANSFER MOLECULAR BEACONS PROBES RNA CHROMOPHORE BINDING
DOI	10.1016/j.polymer.2012.12.080
英文摘要	A simple water-soluble pyrene-functionalized cationic polyelectrolyte poly(4- vinylpyridine) quaternary ammonium (P4VP-Py-Bu) was designed and synthesized from poly(4-vinylpyridine) by two-step quaternization with 1-(bromomethyl)pyrene and 1-bromobutane. The polymer could be bound with a single-stranded DNA (ssDNA) by electrostatic interactions and hydrophobic interactions to form a fluorescent complex probe for the detection of DNA hybridization. When the complementary ssDNA was added into the complex probe solution to form DNA duplex, the fluorescence emission intensity decreased more greatly compared with that by the addition of non-complementary ssDNA. It is inferred that the pyrene moieties of P4VP-Py-Bu could be intercalated into DNA duplex base-pairs, thus the fluorescence quenching by nucleotide bases was strengthened. Both the circular dichroism spectra and the fluorescence quenching induced by iodide confirmed this supposed intercalation. (c) 2013 Elsevier Ltd. All rights reserved.; Polymer Science; SCI(E); EI; 8; ARTICLE; 4; 1289-1294; 54
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/225947]
专题	工学院
推荐引用方式 GB/T 7714	Zhang, Ruichen,Yang, Lingye,Zhao, Min,et al. Synthesis and fluorescence study of a pyrene-functionalized poly(4-vinylpyridine) quaternary ammonium for detection of DNA hybridization[J]. polymer,2013.
APA	Zhang, Ruichen.,Yang, Lingye.,Zhao, Min.,Dong, Jie.,Dong, Haifeng.,...&Wang, Guojie.(2013).Synthesis and fluorescence study of a pyrene-functionalized poly(4-vinylpyridine) quaternary ammonium for detection of DNA hybridization.polymer.
MLA	Zhang, Ruichen,et al."Synthesis and fluorescence study of a pyrene-functionalized poly(4-vinylpyridine) quaternary ammonium for detection of DNA hybridization".polymer (2013).