Metabolic engineering of tomato for high-yield production of astaxanthin

doi:10.1016/j.ymben.2013.02.005

CORC > 北京大学 > 工学院

	Metabolic engineering of tomato for high-yield production of astaxanthin
	Huang, Jun-Chao ; Zhong, Yu-Juan ; Liu, Jin ; Sandmann, Gerhard ; Chen, Feng
刊名	metabolic engineering
	2013
关键词	Astaxanthin beta-carotene ketolase beta-carotene hydroxylase Tomato BETA-CAROTENE KETOLASE HAEMATOCOCCUS-PLUVIALIS ESCHERICHIA-COLI PLANTS BIOSYNTHESIS GENE EXPRESSION PATHWAY FRUIT CANTHAXANTHIN
DOI	10.1016/j.ymben.2013.02.005
英文摘要	Dietary carotenoids have been shown to be beneficial to health by decreasing the risk of many diseases. Attempts to enhance carotenoids in food crops have been successful although higher plants appear to resist big changes of carotenoid biosynthesis by metabolic engineering. Here we report the generation of a more nutritious tomato by modifying the intrinsic carotenes to astaxanthin, a high-value ketocarotenoid rarely found in plants. This was achieved by co-expression of the algal beta-carotene ketolase from Chlamydomonas reinhardtii and beta-carotene hydroxylase from Haematococcus pluvialis, a unique pair of enzymes identified to co-operate perfectly in converting p-carotene to astaxanthin by functional complementation in Escherichia coli. Expression of the two enzymes in tomato up-regulated most intrinsic carotenogenic genes, and efficiently directed carbon flux into carotenoids, leading to massive accumulations of mostly free astaxanthin in leaves (3.12 mg/g) but esterified astaxanthin in fruits (16.1 mg/g) and a 16-fold increase of total carotenoid capacity therein without affecting the plant normal growth and development. This study opened up the possibility of employing crop plants as green factories for economical production of astaxanthin. (C) 2013 Elsevier Inc. All rights reserved.; Biotechnology & Applied Microbiology; SCI(E); EI; 12; ARTICLE; 1; 59-67; 17
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/223903]
专题	工学院
推荐引用方式 GB/T 7714	Huang, Jun-Chao,Zhong, Yu-Juan,Liu, Jin,et al. Metabolic engineering of tomato for high-yield production of astaxanthin[J]. metabolic engineering,2013.
APA	Huang, Jun-Chao,Zhong, Yu-Juan,Liu, Jin,Sandmann, Gerhard,&Chen, Feng.(2013).Metabolic engineering of tomato for high-yield production of astaxanthin.metabolic engineering.
MLA	Huang, Jun-Chao,et al."Metabolic engineering of tomato for high-yield production of astaxanthin".metabolic engineering (2013).