Characteristic microbial community of a dry thermophilic methanogenic   digester: its long-term stability and change with feeding

doi:10.1007/s00253-011-3479-9

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	Characteristic microbial community of a dry thermophilic methanogenic digester: its long-term stability and change with feeding
	Tang, Yue-Qin ; Ji, Pan ; Hayashi, Junpei ; Koike, Yoji ; Wu, Xiao-Lei ; Kida, Kenji
刊名	applied microbiology and biotechnology
	2011
关键词	Thermophilic anaerobic digestion Dry anaerobic digestion Cellulose degradation Garbage digestion Microbial community ORGANIC-SOLID WASTE SP-NOV. ANAEROBIC-DIGESTION GEN-NOV FATTY-ACIDS COMB. NOV BACTERIUM DIVERSITY TEMPERATURE POPULATION
DOI	10.1007/s00253-011-3479-9
英文摘要	Thermophilic dry anaerobic digestion of sludge for cellulose methanization was acclimated at 53 A degrees C for nearly 5 years using a waste paper-based medium. The stability of the microbial community structure and the microbial community responsible for the cellulose methanization were studied by 16S rRNA gene-based clone library analysis. The microbial community structure remained stable during the long-term acclimation period. Hydrogenotrophic methanogens dominated in methanogens and Methanothermobacter, Methanobacterium, Methanoculleus, and Methanosarcina were responsible for the methane production. Bacteria showed relatively high diversity and distributed mainly in the phyla Firmicutes, Bacteroidetes, and Synergistetes. Ninety percent of operational taxonomic units (OTUs) were affiliated with the phylum Firmicutes, indicating the crucial roles of this phylum in the digestion. Relatives of Clostridium stercorarium, Clostridium thermocellum, and Halocella cellulosilytica were dominant cellulose degraders. The acclimated stable sludge was used to treat garbage stillage discharged from a fuel ethanol production process, and the shift of microbial communities with the change of feed was analyzed. Both archaeal and bacterial communities had obviously changed: Methanoculleus spp. and Methanothermobacter spp. and the protein- and fatty acid-degrading bacteria became dominant. Accumulation of ammonia as well as volatile fatty acids led to the inhibition of microbial activity and finally resulted in the deterioration of methane fermentation of the garbage stillage.; Biotechnology & Applied Microbiology; SCI(E); EI; PubMed; 25; ARTICLE; 5; 1447-1461; 91
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/153996]
专题	工学院
推荐引用方式 GB/T 7714	Tang, Yue-Qin,Ji, Pan,Hayashi, Junpei,et al. Characteristic microbial community of a dry thermophilic methanogenic digester: its long-term stability and change with feeding[J]. applied microbiology and biotechnology,2011.
APA	Tang, Yue-Qin,Ji, Pan,Hayashi, Junpei,Koike, Yoji,Wu, Xiao-Lei,&Kida, Kenji.(2011).Characteristic microbial community of a dry thermophilic methanogenic digester: its long-term stability and change with feeding.applied microbiology and biotechnology.
MLA	Tang, Yue-Qin,et al."Characteristic microbial community of a dry thermophilic methanogenic digester: its long-term stability and change with feeding".applied microbiology and biotechnology (2011).