Gold nanoparticles-decorated graphene field-effect transistor biosensor   for femtomolar MicroRNA detection

doi:10.1016/j.bios.2015.06.068

CORC > 北京大学 > 信息科学技术学院

	Gold nanoparticles-decorated graphene field-effect transistor biosensor for femtomolar MicroRNA detection
	Cai, Bingjie ; Huang, Le ; Zhang, Hong ; Sun, Zhongyue ; Zhang, Zhiyong ; Zhang, Guo-Jun
刊名	BIOSENSORS & BIOELECTRONICS
	2015
关键词	MicroRNA detection Graphene Gold nanoparticles Field-effect transistor biosensor Peptide nucleic acid SILICON NANOWIRE BIOSENSORS LABEL-FREE MICRORNAS DNA HYBRIDIZATION PROTEIN-DETECTION OXIDE EXPRESSION SENSORS BINDING MIRNAS CANCER
DOI	10.1016/j.bios.2015.06.068
英文摘要	Early detection is proven to be the best chance for successful cancer treatment. MiRNAs, as ideal biomarkers, can identify cancer in the early stage. Therefore, development of highly sensitive and selective detection methods for miRNA is still anticipated. Here we report on a gold nanoparticles (AuNPs)-decorated graphene field-effect transistor (FET) biosensor for highly sensitive, selective and label-free detection of miRNA. The AuNPs-decorated graphene FET biosensor was fabricated by drop-casting the reduced graphene oxide (R-GO) suspension onto the sensor surface, and subsequently decorating AuNPs onto the surface of R-GO. After peptide nucleic acid (PNA) probe was immobilized on the AuNPs surface, miRNA detection was carried out via PNA-miRNA hybridization. It was found that the developed FET biosensor was able to achieve a detection limit as low as 10 fM. In addition, the biosensor enabled an accurate distinction of complementary miRNA from one-base mismatched miRNA and non-complementary miRNA. What's more, this highly sensitive and selective assay was also applied to the detection of miRNA in serum samples, making it a potential method for diagnosis of gene-related diseases. (C) 2015 Elsevier B.V. All rights reserved.; National Natural Science Foundation of China [21275040, 21475034]; Natural Science Foundation of Hubei Province [2013CFA061]; SCI(E); EI; PubMed; ARTICLE; zyzhang@pku.edu.cn; zhanggj@hbtcm.edu.cn; 329-334; 74
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/415216]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Cai, Bingjie,Huang, Le,Zhang, Hong,et al. Gold nanoparticles-decorated graphene field-effect transistor biosensor for femtomolar MicroRNA detection[J]. BIOSENSORS & BIOELECTRONICS,2015.
APA	Cai, Bingjie,Huang, Le,Zhang, Hong,Sun, Zhongyue,Zhang, Zhiyong,&Zhang, Guo-Jun.(2015).Gold nanoparticles-decorated graphene field-effect transistor biosensor for femtomolar MicroRNA detection.BIOSENSORS & BIOELECTRONICS.
MLA	Cai, Bingjie,et al."Gold nanoparticles-decorated graphene field-effect transistor biosensor for femtomolar MicroRNA detection".BIOSENSORS & BIOELECTRONICS (2015).